About: A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners

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About: A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.linkos.cz/files/klinicka-onkologie/174/4133.pdf
Description	Expression of p63 is essential for the formation of epidermis and other stratifying epithelia. Moreover p63 is highly expressed in several epithelial cancers and is involved in tumourigenesis and controlling chemo-sensitivity. The identification of p63 interacting partners is essential for understanding the complex network of gene regulation managing epithelial development and could also help to reveal signalling pathways participating in UV-damage response in human skin. We used a proteomic approach to identify proteins that interact with ΔNp63. Proteins were isolated by immunoprecipitation with ΔNp63 specific antibody and analysed by mass spectrometry. We identified 23 proteins as potential ΔNp63 binding partners that were not present in negative control samples. These results will be evaluated using other methods. Expression of p63 is essential for the formation of epidermis and other stratifying epithelia. Moreover p63 is highly expressed in several epithelial cancers and is involved in tumourigenesis and controlling chemo-sensitivity. The identification of p63 interacting partners is essential for understanding the complex network of gene regulation managing epithelial development and could also help to reveal signalling pathways participating in UV-damage response in human skin. We used a proteomic approach to identify proteins that interact with ΔNp63. Proteins were isolated by immunoprecipitation with ΔNp63 specific antibody and analysed by mass spectrometry. We identified 23 proteins as potential ΔNp63 binding partners that were not present in negative control samples. These results will be evaluated using other methods. (en)
Title	A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners (en)
skos:prefLabel	A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners A combined immunoprecipitation and mass spectrometric approach to determine ΔNp63-interacting partners (en)
skos:notation	RIV/00209805:_____/12:#0000305!RIV13-MSM-00209805
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED2.1.00/03.0101), P(GBP206/12/G151), P(GPP301/10/P431)
http://linked.open...iv/cisloPeriodika	supplementum 2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Hernychová, Lenka Vojtěšek, Bořivoj Nekulová, Marta Holčáková, Jitka Michalová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	120071
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/12:#0000305
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antibodies; p63; immunoprecipitation; proteomics; mass spectrometry; protein-protein interactions (en)
http://linked.open.../riv/klicoveSlovo	antibodies p63 immunoprecipitation protein-protein interactions mass spectrometry proteomics
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[2C9258ECC48D]
http://linked.open...i/riv/nazevZdroje	Klinická onkologie
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of p63 expression in human epithelial cells Center of novel approaches to bioanalysis and molecular diagnostics Regional Centre for Applied Molecular Oncology (RECAMO)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Hernychová, Lenka Vojtěšek, Bořivoj Holčáková, Jitka Michalová, Eva Nekulová, Miroslava
issn	0862-495X
number of pages	6 (xsd:int)

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