About: Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry

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About: Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present study, a series of 119 human cancers were immunostained using a highly sensitive technique that detects the low levels of wild-type protein expressed in normal cells. TP53 gene status was also assessed using FASAY and in selected cases by direct sequencing. The transcriptional activity of TP53 was determined by staining for two downstream target genes, p21(WAF1) and MDM2, and statistical correlations between MDM2/p21(WAF1) and p53 were found in tumours with wild-type, but not mutant TP53. Measurement of staining for p53 and MDM2 accurately identifies the TP53 status of tumours. In the present study, a series of 119 human cancers were immunostained using a highly sensitive technique that detects the low levels of wild-type protein expressed in normal cells. TP53 gene status was also assessed using FASAY and in selected cases by direct sequencing. The transcriptional activity of TP53 was determined by staining for two downstream target genes, p21(WAF1) and MDM2, and statistical correlations between MDM2/p21(WAF1) and p53 were found in tumours with wild-type, but not mutant TP53. Measurement of staining for p53 and MDM2 accurately identifies the TP53 status of tumours. (en) V prezentované studii jsme provedli imunohistochemickou analýzu 119 lidských nádorů s využitím velmi senzitivní metody, která umožnila detekovat velmi nízké hladiny nemutované formy p53 u normálních buněk. Status genu TP53 byl rovněž analyzován pomocí metody FASAY a ve vybraných případech robvněž přímým sekvenováním genu TP53. Transkripční aktivita p53 byla stanovena imunohistochemickou detekcí dvou cílových genů p21(WAF1) a MDM2. Byly nalezeny statistické korelace mezi expresí MDM2/p21(WAF1) a p53 pouze u nádorů s nemutovanou formou p53.Analýza barvení p53 a MDM2 přesně identifikuje status TP53 u nádorů. (cs)
Title	Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry (en) Rozlišení funkčního a nefunkčního p53 u lidských nádorů s využitím immunohistochemické detekce p53 a MDM2 (cs)
skos:prefLabel	Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry Discriminating functional and non-functional p53 in human tumours by p53 and MDM2 immunohistochemistry (en) Rozlišení funkčního a nefunkčního p53 u lidských nádorů s využitím immunohistochemické detekce p53 a MDM2 (cs)
skos:notation	RIV/00209805:_____/05:#0000024!RIV06-MZ0-00209805
http://linked.open.../vavai/riv/strany	251; 259
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NC7131), P(NR8338)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Müller, Petr Vojtěšek, Bořivoj Nenutil, Rudolf
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	518246
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/05:#0000024
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	P53, MDM2, immunohistochemistry, TP53 mutation, p53 phosphorylation (en)
http://linked.open.../riv/klicoveSlovo	TP53 mutation immunohistochemistry MDM2 p53 phosphorylation P53
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[50126C465BC4]
http://linked.open...i/riv/nazevZdroje	Journal of Pathology
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of functional alterations of mutant forms of p53 protein in human cancers. Functional analysis of the p53-dependent pathways in breast cancer: Integration of genomic and proteomic approaches using DNA-macroarray and SELDI-TOF mass spectrometry techniques
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	207
http://linked.open...iv/tvurceVysledku	Vojtěšek, Bořivoj Müller, Petr Nenutil, Rudolf
issn	0022-3417
number of pages	9 (xsd:int)

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