About: Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients     Goto   Sponge   NotDistinct   Permalink

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Description
  • To assess the clinical validity of polymerase chain reaction (PCR) based molecular methods in the microbiological diagnosis of culture negative infective endocarditis in a group of surgically treated patients. Retrospective case-control study. Reference cardiovascular surgical centre. 15 culture negative patients with infective endocarditis classified according to Duke criteria, with 17 heart valve samples; 13 age and sex matched control patients without infective endocarditis, with 13 valve samples. Medical records were reviewed and clinical, demographic, and microbiological data collected, including results of molecular detection of bacteria and fungi from valve samples. The clinical validity of molecular diagnosis was assessed, along with the sensitivity and speed of the systems. In the study group, 14 patients were PCR positive (93%). Organisms detected were streptococci (3), staphylococci (2), enterobacter (1), Tropheryma whippelii (1), Borrelia burgdorferi (1), Candida albicans (1), and Aspergillus species (2). Three cases were positive on universal bacterial detection but the pathogen could not be identified because of contaminating background. One case was negative. All but two positive cases showed clinical correlations. These two cases had no symptoms of infective endocarditis but there was agreement with the surgical findings. All control cases were PCR negative. Results were available within eight hours, and if sequencing was necessary, within 48 hours. PCR based molecular detection of pathogens in valve samples from surgically treated culture negative infective endocarditis patients is fast, and reliable. The technology, combined with thorough validation and clinical interpretation, may be a promising tool for routine testing of infective endocarditis.
  • To assess the clinical validity of polymerase chain reaction (PCR) based molecular methods in the microbiological diagnosis of culture negative infective endocarditis in a group of surgically treated patients. Retrospective case-control study. Reference cardiovascular surgical centre. 15 culture negative patients with infective endocarditis classified according to Duke criteria, with 17 heart valve samples; 13 age and sex matched control patients without infective endocarditis, with 13 valve samples. Medical records were reviewed and clinical, demographic, and microbiological data collected, including results of molecular detection of bacteria and fungi from valve samples. The clinical validity of molecular diagnosis was assessed, along with the sensitivity and speed of the systems. In the study group, 14 patients were PCR positive (93%). Organisms detected were streptococci (3), staphylococci (2), enterobacter (1), Tropheryma whippelii (1), Borrelia burgdorferi (1), Candida albicans (1), and Aspergillus species (2). Three cases were positive on universal bacterial detection but the pathogen could not be identified because of contaminating background. One case was negative. All but two positive cases showed clinical correlations. These two cases had no symptoms of infective endocarditis but there was agreement with the surgical findings. All control cases were PCR negative. Results were available within eight hours, and if sequencing was necessary, within 48 hours. PCR based molecular detection of pathogens in valve samples from surgically treated culture negative infective endocarditis patients is fast, and reliable. The technology, combined with thorough validation and clinical interpretation, may be a promising tool for routine testing of infective endocarditis. (en)
Title
  • Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients
  • Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients (en)
skos:prefLabel
  • Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients
  • Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients (en)
skos:notation
  • RIV/00209775:_____/03:#0000254!RIV13-MZ0-00209775
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(NM6870), Z(MZ0L010209775)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 616267
http://linked.open...ai/riv/idVysledku
  • RIV/00209775:_____/03:#0000254
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • prosthetic valve endocarditis; PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [7164ABFFC109]
http://linked.open...i/riv/nazevZdroje
  • Heart
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 89
http://linked.open...iv/tvurceVysledku
  • Černý, Jan
  • Dendis, Miloš
  • Horváth, Radek
  • Benedík, Jaroslav
  • Grijalva, Marcelo
http://linked.open...ain/vavai/riv/wos
  • 000181276000013
http://linked.open...n/vavai/riv/zamer
issn
  • 1355-6037
number of pages
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