About: Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia

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About: Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1179/1607845412Y.0000000015
Description	Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL. Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL. (en)
Title	Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia (en)
skos:prefLabel	Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia (en)
skos:notation	RIV/00179906:_____/12:10124568!RIV13-MZ0-00179906
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(NT13412)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Krejsek, Jan Smolej, Lukáš Vroblová, Vladimíra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Hradec Králové
http://linked.open...dnocenehoVysledku	158766
http://linked.open...ai/riv/idVysledku	RIV/00179906:_____/12:10124568
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Standardization; Prognosis; Flow cytometry; CLL; ZAP-70 (en)
http://linked.open.../riv/klicoveSlovo	Standardization ZAP-70 CLL Prognosis Flow cytometry
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[BC1732549AC5]
http://linked.open...i/riv/nazevZdroje	Hematology
http://linked.open...in/vavai/riv/obor	FN
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Complex assessment of microenvironment and its impact on clinical course of chronic lymphocytic leukemia.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Krejsek, Jan Smolej, Lukáš Vroblová, Vladimíra
http://linked.open...ain/vavai/riv/wos	000308350000004
issn	1024-5332
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1179/1607845412Y.0000000015

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