About: Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

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About: Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0039914011005121
Description	A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. (en)
Title	Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC (en)
skos:prefLabel	Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC (en)
skos:notation	RIV/00179906:_____/11:10100325!RIV12-MZ0-00179906
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021620820), Z(MSM0021620822), Z(MZ0FNHK2005)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Krčmová, Lenka Solichová, Dagmar Sobotka, Luboš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Hradec Králové
http://linked.open...dnocenehoVysledku	193853
http://linked.open...ai/riv/idVysledku	RIV/00179906:_____/11:10100325
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Clinical research; Monolithic column; HPLC; Tryptophan; Kynurenine; Neopterin (en)
http://linked.open.../riv/klicoveSlovo	Tryptophan Clinical research Kynurenine Monolithic column Neopterin HPLC
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BF30932E48D9]
http://linked.open...i/riv/nazevZdroje	Talanta
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	85
http://linked.open...iv/tvurceVysledku	Kašparová, Markéta Krčmová, Lenka Melichar, Bohuslav Plíšek, Jiří Sobotka, Luboš Solich, Petr Solichová, Dagmar
http://linked.open...ain/vavai/riv/wos	000294092800038
http://linked.open...n/vavai/riv/zamer	The use of experimental and clinical models of metabolic processes, nutrition and pharmacotherapy for the advancement of knowledge, clinical practice and quality of life improvement Nové diagnostické indikátory a léčebné postupy v závislosti na různých obdobích života s důrazem na stáří Research of new drug structures
issn	0039-9140
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.talanta.2011.06.027

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