About: LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas

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About: LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study is to demostrate possibility of detection of Ki-ras with a synthetic nucleotide analogue, locked nucleic acid (LNA).We consider LNA-clamped real-time PCR to be a fast alternative method for specific analysis of the Ki-ras gene in colorectal carcinomas. The reliability determined in CRC tissue was comparable with the enriched PCR/RFLP and nucleotide sequencing. The aim of this study is to demostrate possibility of detection of Ki-ras with a synthetic nucleotide analogue, locked nucleic acid (LNA).We consider LNA-clamped real-time PCR to be a fast alternative method for specific analysis of the Ki-ras gene in colorectal carcinomas. The reliability determined in CRC tissue was comparable with the enriched PCR/RFLP and nucleotide sequencing. (en) Cílem studie je demonstrovat možnost detekce mutací v Ki-ras genu pomocí metody LNA-clamping PCR. Dle dosažených výsledků považujeme tuto metodu za velmi rychlou alternativu pro specifickou analýzu přítomnosti mutací v Ki-ras genu u kolorektálních karcinomů. (cs)
Title	LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas (en) LNA clampovací PCR: Specifická metoda pro detekci mutací Ki-ras genů u pacientů se sporadickými kolorektálními karcinomy (cs)
skos:prefLabel	LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas LNA clamped PCR: A specific method for detection of Ki-ras gene mutations in patients with sporadic colorectal carcinomas (en) LNA clampovací PCR: Specifická metoda pro detekci mutací Ki-ras genů u pacientů se sporadickými kolorektálními karcinomy (cs)
skos:notation	RIV/00179906:_____/06:00000023!RIV07-MZ0-00179906
http://linked.open.../vavai/riv/strany	217;220
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NR7884)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Beránek, Martin Živný, Pavel Jandík, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Hradec Králové
http://linked.open...dnocenehoVysledku	483480
http://linked.open...ai/riv/idVysledku	RIV/00179906:_____/06:00000023
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ki-ras gene; point mutations; LNA; DNA; PCR; colorectal carcinoma; plasma (en)
http://linked.open.../riv/klicoveSlovo	DNA PCR plasma LNA point mutations colorectal carcinoma ki-ras gene
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[82E59E80A4C0]
http://linked.open...i/riv/nazevZdroje	Klinická biochemie a metabolismus
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of the prognossis and treatment patients with CRC to find out of the circulated tumor cells in assitance with PCR of the K-ras mutation
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Beránek, Martin Živný, Pavel Jandík, Pavel
issn	1210-7921
number of pages	4 (xsd:int)

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