About: Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://fb.cuni.cz/file/5751/fb2014a0038.pdf
Description	Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this short communication, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34(+) blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions. Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this short communication, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34(+) blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions. (en)
Title	Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods (en)
skos:prefLabel	Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods (en)
skos:notation	RIV/00159816:_____/14:00061217!RIV15-MSM-00159816
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.100/02/0123), P(GBP302/12/G157)
http://linked.open...iv/cisloPeriodika	S1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Paďourová, Stanislava Šimara, Pavel Krontorád Koutná, Irena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice u sv.Anny v Brně
http://linked.open...dnocenehoVysledku	18058
http://linked.open...ai/riv/idVysledku	RIV/00159816:_____/14:00061217
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	episomal reprogramming; Sendai virus; lentivirus; hiPSCs (en)
http://linked.open.../riv/klicoveSlovo	Sendai virus episomal reprogramming hiPSCs lentivirus
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[8F9B89DA70A3]
http://linked.open...i/riv/nazevZdroje	Folia biologica (Praha)
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Šimara, Pavel Krontorád Koutná, Irena Paďourová, Stanislava Tesařová, L.
http://linked.open...ain/vavai/riv/wos	000343275800014
issn	0015-5500
number of pages	5 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software