About: Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:246-254 The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:246-254 (en)
Title	Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability (en)
skos:prefLabel	Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability (en)
skos:notation	RIV/00159816:_____/13:00060810!RIV14-MZ0-00159816
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED1.100/02/0123), P(GBP302/12/G157), P(LC06077), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hampl, Aleš Dvořák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice u sv.Anny v Brně
http://linked.open...dnocenehoVysledku	59468
http://linked.open...ai/riv/idVysledku	RIV/00159816:_____/13:00060810
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Induced pluripotent stem cells; Cell survival; Xenograft assay; Cell culture; Embryonic stem cells (en)
http://linked.open.../riv/klicoveSlovo	Cell survival Induced pluripotent stem cells Xenograft assay Embryonic stem cells Cell culture
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C3EE75815538]
http://linked.open...i/riv/nazevZdroje	Stem Cells Translational Medicine
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions Center of Chemical Genetics St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	2
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Hampl, Aleš Kyrylenko, Sergiy Eiselleova, Livia Kubikova, Iva Kunova, Michaela Matulka, Kamil Salykin, Anton
http://linked.open...ain/vavai/riv/wos	000317551800002
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	2157-6564
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.5966/sctm.2012-0081

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