About: Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis

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About: Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	?d T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the ? variable (V?) and d variable (Vd) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region spectratyping, but a fast and simple assessment of V? and Vd gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the ?d T-cell population. This report provides optimized PCR conditions for ?d T-cell receptor amplification. Further, it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of V? and Vd gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during haemato-oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the ?d T-cell population composition in tissues of interest. ?d T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the ? variable (V?) and d variable (Vd) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region spectratyping, but a fast and simple assessment of V? and Vd gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the ?d T-cell population. This report provides optimized PCR conditions for ?d T-cell receptor amplification. Further, it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of V? and Vd gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during haemato-oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the ?d T-cell population composition in tissues of interest. (en)
Title	Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis (en)
skos:prefLabel	Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis (en)
skos:notation	RIV/00159816:_____/12:#0000957!RIV13-MSM-00159816
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED1.100/02/0123), P(NS9681), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Krontorád Koutná, Irena Klabusay, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice u sv.Anny v Brně
http://linked.open...dnocenehoVysledku	164873
http://linked.open...ai/riv/idVysledku	RIV/00159816:_____/12:#0000957
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PERIPHERAL-BLOOD (en)
http://linked.open.../riv/klicoveSlovo	PERIPHERAL-BLOOD
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D3E8EAB32D9B]
http://linked.open...i/riv/nazevZdroje	INTERNATIONAL JOURNAL OF IMMUNOGENETICS
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Studying molecular biologic responses to intensive insulin therapy during lung resections St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	39
http://linked.open...iv/tvurceVysledku	Klabusay, Martin Koutná, Irena
http://linked.open...ain/vavai/riv/wos	000308406600002
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	1744-3121
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1744-313X.2012.01114.x

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