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rdf:type
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Description
| - ?d T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the ? variable (V?) and d variable (Vd) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region spectratyping, but a fast and simple assessment of V? and Vd gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the ?d T-cell population. This report provides optimized PCR conditions for ?d T-cell receptor amplification. Further, it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of V? and Vd gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during haemato-oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the ?d T-cell population composition in tissues of interest.
- ?d T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the ? variable (V?) and d variable (Vd) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region spectratyping, but a fast and simple assessment of V? and Vd gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the ?d T-cell population. This report provides optimized PCR conditions for ?d T-cell receptor amplification. Further, it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of V? and Vd gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during haemato-oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the ?d T-cell population composition in tissues of interest. (en)
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Title
| - Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis
- Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis (en)
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skos:prefLabel
| - Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis
- Relative expression of gamma delta T-cell receptor gene families detected by RT-PCR and capillary electrophoresis (en)
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skos:notation
| - RIV/00159816:_____/12:#0000957!RIV13-MSM-00159816
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http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(ED1.100/02/0123), P(NS9681), Z(MSM0021622430)
|
http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00159816:_____/12:#0000957
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| |
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| - INTERNATIONAL JOURNAL OF IMMUNOGENETICS
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Klabusay, Martin
- Koutná, Irena
|
http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
| |
http://bibframe.org/vocab/doi
| - 10.1111/j.1744-313X.2012.01114.x
|