About: PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak

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About: PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.jcf.2012.04.005
Description	Background: Once the outbreak with Burkholderia cenocepacia ST32 was identified in the Prague cystic fibrosis (CF) centre, molecular tools were implemented into diagnostic routine in order to complement infection control measures with as accurate as possible microbiological service. In addition, genotyping techniques were applied as part of an infection surveillance program to assign species and strain status in samples positive for Burkholderia cepacia complex (Bcc). We sought to evaluate a usefulness of Bcc-specific PCR in infection control and to map evolution of the outbreak. Methods: Since 2001, 6109 respiratory samples from 299 CF patients were examined not only by conventional culture, but also by PCR, detecting Bcc directly in sputum. Results: Diagnosis of Bcc infection was established by culture in 54 patients already prior to 2001. As 39 more patients were diagnosed by culture and/or PCR during 2001-2010, this represented annual prevalence of 18.5%-28.9%. Twelve of 39 patients were culture negative at the time of their first PCR positivity. Although 2/3 of them became subsequently culture positive, the time delay in diagnostics of the infection by culture ranged from 1 to 22 months. New cases of Bcc infection were detected every year, however a dramatic drop was observed for the epidemic strain ST32. Conclusion: A likely factor contributing to the end of ST32 epidemic was segregation of Bcc infected patients that included also patients with no culture, but PCR positivity. The diagnostic PCR led to timely identification of cases with 'culture-invisible' infection. Background: Once the outbreak with Burkholderia cenocepacia ST32 was identified in the Prague cystic fibrosis (CF) centre, molecular tools were implemented into diagnostic routine in order to complement infection control measures with as accurate as possible microbiological service. In addition, genotyping techniques were applied as part of an infection surveillance program to assign species and strain status in samples positive for Burkholderia cepacia complex (Bcc). We sought to evaluate a usefulness of Bcc-specific PCR in infection control and to map evolution of the outbreak. Methods: Since 2001, 6109 respiratory samples from 299 CF patients were examined not only by conventional culture, but also by PCR, detecting Bcc directly in sputum. Results: Diagnosis of Bcc infection was established by culture in 54 patients already prior to 2001. As 39 more patients were diagnosed by culture and/or PCR during 2001-2010, this represented annual prevalence of 18.5%-28.9%. Twelve of 39 patients were culture negative at the time of their first PCR positivity. Although 2/3 of them became subsequently culture positive, the time delay in diagnostics of the infection by culture ranged from 1 to 22 months. New cases of Bcc infection were detected every year, however a dramatic drop was observed for the epidemic strain ST32. Conclusion: A likely factor contributing to the end of ST32 epidemic was segregation of Bcc infected patients that included also patients with no culture, but PCR positivity. The diagnostic PCR led to timely identification of cases with 'culture-invisible' infection. (en)
Title	PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak (en)
skos:prefLabel	PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak (en)
skos:notation	RIV/00064203:_____/12:8263!RIV13-MZ0-00064203
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NS10543), P(NT12405)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Cinek, Ondřej Dřevínek, Pavel Vávrová, Věra Fila, Libor Bartošová, Jana Zemková, Daniela Skalická, Veronika Melter, Oto Kučerová, Tereza Kalferstová, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice v Motole
http://linked.open...dnocenehoVysledku	158027
http://linked.open...ai/riv/idVysledku	RIV/00064203:_____/12:8263
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cystic-fibrosis; sp-nov; epidemiology; cenocepacia; infection; identification; diversity; provides; strains; dna (en)
http://linked.open.../riv/klicoveSlovo	diversity epidemiology identification infection dna strains cenocepacia provides sp-nov cystic-fibrosis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A4CDC1B929BC]
http://linked.open...i/riv/nazevZdroje	Journal of Cystic Fibrosis
http://linked.open...in/vavai/riv/obor	FC
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular identification of bacterial strains which cause severe epidemics in Czech patients with cystic fibrosis and prevention of their spread within this population Pathogenesis of fatal infection complication in patients with cystic fibrosis which is caused by Burkholderia cenocepacia
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Cinek, Ondřej Dřevínek, Pavel Vávrová, Věra Fila, Libor Bartošová, Jana Skalická, Veronika Zemková, Daniela Melter, Oto Dědečková, Klára Kalferstová, Lucie Kučerová, Tereza
http://linked.open...ain/vavai/riv/wos	000309431800012
issn	1569-1993
number of pages	6 (xsd:int)

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