About: Zeptomole Electrochemical Detection of Metallothioneins

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument. We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument. (en)
Title	Zeptomole Electrochemical Detection of Metallothioneins Zeptomole Electrochemical Detection of Metallothioneins (en)
skos:prefLabel	Zeptomole Electrochemical Detection of Metallothioneins Zeptomole Electrochemical Detection of Metallothioneins (en)
skos:notation	RIV/00064203:_____/10:6272!RIV11-MZ0-00064203
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(IAA401990701)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Eckschlager, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice v Motole
http://linked.open...dnocenehoVysledku	299929
http://linked.open...ai/riv/idVysledku	RIV/00064203:_____/10:6272
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	plasma-mass spectrometry; cancer; chronopotentiometry; speciation; proteomics; proteins; level (en)
http://linked.open.../riv/klicoveSlovo	plasma-mass spectrometry cancer level proteins proteomics chronopotentiometry speciation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[ECC53BD87802]
http://linked.open...i/riv/nazevZdroje	PLoS One
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Adam, V. Eckschlager, Tomáš Kizek, R. Wang, J. Petrlova, J. Trnková, L.
http://linked.open...ain/vavai/riv/wos	000279522500009
issn	1932-6203
number of pages	8 (xsd:int)

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