About: A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma

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About: A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A clinical course of patients with mantle cell lymphoma (MCL) is aggressive, and the disease is rarely curable. Proliferation rate is the most important prognostic factor. We developed a novel, reliable, rapid, and routinely applicable approach allowing a precise quantitative assessment of three proliferation markers, Ki-67, topoisomerase II alpha, and TPX2. We employed real-time reverse transcription PCR (RQ-RT-PCR). We observed individual variability of the mRNA expression levels, reflecting heterogeneity of the proliferation rate in individual patients. In general, we observed the highest mRNA expression in the group of Burkitt lymphomas and the lowest in patients with reactive lymphadenopathies. We found increased proliferation rate in MCLs with high cyclin D1 mRNA indicating a quantitative cell cycle control. We observed a correlation between mRNA level and the immunohistochemical staining of corresponding proteins which significantly argues for the prognostic significance of the mRNA measuring. A clinical course of patients with mantle cell lymphoma (MCL) is aggressive, and the disease is rarely curable. Proliferation rate is the most important prognostic factor. We developed a novel, reliable, rapid, and routinely applicable approach allowing a precise quantitative assessment of three proliferation markers, Ki-67, topoisomerase II alpha, and TPX2. We employed real-time reverse transcription PCR (RQ-RT-PCR). We observed individual variability of the mRNA expression levels, reflecting heterogeneity of the proliferation rate in individual patients. In general, we observed the highest mRNA expression in the group of Burkitt lymphomas and the lowest in patients with reactive lymphadenopathies. We found increased proliferation rate in MCLs with high cyclin D1 mRNA indicating a quantitative cell cycle control. We observed a correlation between mRNA level and the immunohistochemical staining of corresponding proteins which significantly argues for the prognostic significance of the mRNA measuring. (en)
Title	A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma (en)
skos:prefLabel	A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma (en)
skos:notation	RIV/00064203:_____/10:6243!RIV11-MZ0-00064203
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MZ0FNM2005)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kodet, Roman Mrhalová, Marcela Krsková, Lenka Břízová, Helena Kalinová, Markéta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice v Motole
http://linked.open...dnocenehoVysledku	244835
http://linked.open...ai/riv/idVysledku	RIV/00064203:_____/10:6243
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Real-time PCR; Proliferation markers; Ki-67; Topoisomerase II alpha; TPX2; Mantle cell lymphoma; Mol Pathol; Quantitative Path; Lymphoma; cyclin d1 overexpression; european-mcl-network; prognostic-factors; messenger-rna; expression; survival; features; protein; gene; identification (en)
http://linked.open.../riv/klicoveSlovo	prognostic-factors features Ki-67 Mantle cell lymphoma expression gene identification protein survival messenger-rna Lymphoma Real-time PCR Mol Pathol Proliferation markers Quantitative Path TPX2 Topoisomerase II alpha cyclin d1 overexpression european-mcl-network
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[52B219BFB4D7]
http://linked.open...i/riv/nazevZdroje	Virchows Archiv
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	456
http://linked.open...iv/tvurceVysledku	Kodet, Roman Mrhalová, Marcela Břízová, Helena Kalinová, Markéta Krsková, Lenka
http://linked.open...ain/vavai/riv/wos	000278363800010
http://linked.open...n/vavai/riv/zamer	Zdokonalení časné diagnostiky, prevence a léčby těžkých vrozených vývojových vad a chorob geneticky podmíněných či s genetickou dispozicí v prenatální a postnatální fázi vývoje
issn	0945-6317
number of pages	9 (xsd:int)

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