About: Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

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About: Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8) to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of MT determined. We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on MT signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. If we compared the GSH and MT signals at the accumulation time of 120 s, the MT signal was more than two hundred times higher than the GSH one. The influence of Tween-20 was investigated in a broad concentration range (from 10(-8) to 10(-11) %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10(-3) - 10(-6) M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblasts sample and recalculated the concentration of MT determined. (en)
Title	Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction (en)
skos:prefLabel	Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction (en)
skos:notation	RIV/00064203:_____/09:5515!RIV10-AV0-00064203
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA401990701), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	3-5
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Průša, Richard Kukačka, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice v Motole
http://linked.open...dnocenehoVysledku	312430
http://linked.open...ai/riv/idVysledku	RIV/00064203:_____/09:5515
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Differential pulse voltammetry; Adsorptive transfer stripping technique; Proteins; Metallothionein; Brdicka reaction; current stripping chronopotentiometry; heavy-metal ions; electrochemical-behavior; mercury-electrode; expression; biosensor; melanoma; level; cells (en)
http://linked.open.../riv/klicoveSlovo	Metallothionein Proteins Differential pulse voltammetry expression level Adsorptive transfer stripping technique biosensor cells Brdicka reaction melanoma current stripping chronopotentiometry electrochemical-behavior heavy-metal ions mercury-electrode
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[7DA12B01AF50]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Adam, V. Kizek, R. Průša, Richard Kukačka, Jiří Fabrik, I. Krizkova, S. Trnková, L. Strnadel, J. Horak, V.
http://linked.open...ain/vavai/riv/wos	000264063500057
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	1040-0397
number of pages	5 (xsd:int)

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