About: Allergenicity of main birch allergen rBet v1 and high-pressure treatment

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About: Allergenicity of main birch allergen rBet v1 and high-pressure treatment Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of our study was to determine the influence of high-pressure treatment on the structure and allergenicity of recombinant rBet v1, the main birch allergen and birch pollen extract. We treated the buffered solutions of rBet v1 and birch pollen extract with high pressure (450-550MPa) for a period of 10 min at temperatures between 30 and 50 C. The structural changes in rBet v1 were studied using circular dichroism spectra. The greatest changes in the rBet v1 structure were found in the samples treated at 450MPa at 30 C. The samples treated at 500 and 550MPa at 30 C (10 min) did not show any structural changes. The allergenic reaction to rBet v1 and the birch pollen extract was tested using Western blot analysis. Pressures of 450-550MPa (10 min) at temperatures of 30, 40 and 50 C did not change the allergenicity of the rBet v1 protein or the birch pollen extract when compared with untreated samples. The aim of our study was to determine the influence of high-pressure treatment on the structure and allergenicity of recombinant rBet v1, the main birch allergen and birch pollen extract. We treated the buffered solutions of rBet v1 and birch pollen extract with high pressure (450-550MPa) for a period of 10 min at temperatures between 30 and 50 C. The structural changes in rBet v1 were studied using circular dichroism spectra. The greatest changes in the rBet v1 structure were found in the samples treated at 450MPa at 30 C. The samples treated at 500 and 550MPa at 30 C (10 min) did not show any structural changes. The allergenic reaction to rBet v1 and the birch pollen extract was tested using Western blot analysis. Pressures of 450-550MPa (10 min) at temperatures of 30, 40 and 50 C did not change the allergenicity of the rBet v1 protein or the birch pollen extract when compared with untreated samples. (en)
Title	Allergenicity of main birch allergen rBet v1 and high-pressure treatment Allergenicity of main birch allergen rBet v1 and high-pressure treatment (en)
skos:prefLabel	Allergenicity of main birch allergen rBet v1 and high-pressure treatment Allergenicity of main birch allergen rBet v1 and high-pressure treatment (en)
skos:notation	RIV/00064173:_____/09:00002248!RIV13-MZ0-00064173
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(2B06139), V
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Vávrová, Helena Kučera, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Královské Vinohrady
http://linked.open...dnocenehoVysledku	302500
http://linked.open...ai/riv/idVysledku	RIV/00064173:_____/09:00002248
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	high pressure; allergen; rBet v1; birch pollen extract (en)
http://linked.open.../riv/klicoveSlovo	allergen birch pollen extract rBet v1 high pressure
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[D6C4D59CC978]
http://linked.open...i/riv/nazevZdroje	High Pressure Research
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Inactivation alergens in foods by the high pressure treatment
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Kučera, Petr Šetinová, Ivana Vávrová, Helena
http://linked.open...ain/vavai/riv/wos	000274675700041
issn	0895-7959
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/08957950903375055

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