About: Allergenicity of main celery allergen rApi g1 and high-pressure treatment

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500MPa for pressure holding times 10 and 20min and holding time temperatures 30 degrees C, 40 degrees C and 50 degrees C. The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis. The greatest changes of the structure were found at samples treated by 500MPa at 50 degrees C. The samples treated at this temperature at pressure levels 400, 450 and 500MPa and held for 10 and 20min showed that protein structure changes are positively correlated with pressure. Western blot analysis evidenced that a pressure of 500MPa held for 10-20min at temperatures 30-50 degrees C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample. The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500MPa for pressure holding times 10 and 20min and holding time temperatures 30 degrees C, 40 degrees C and 50 degrees C. The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis. The greatest changes of the structure were found at samples treated by 500MPa at 50 degrees C. The samples treated at this temperature at pressure levels 400, 450 and 500MPa and held for 10 and 20min showed that protein structure changes are positively correlated with pressure. Western blot analysis evidenced that a pressure of 500MPa held for 10-20min at temperatures 30-50 degrees C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample. (en)
Title	Allergenicity of main celery allergen rApi g1 and high-pressure treatment Allergenicity of main celery allergen rApi g1 and high-pressure treatment (en)
skos:prefLabel	Allergenicity of main celery allergen rApi g1 and high-pressure treatment Allergenicity of main celery allergen rApi g1 and high-pressure treatment (en)
skos:notation	RIV/00064173:_____/09:00002247!RIV13-MZ0-00064173
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(2B06139), V
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Vávrová, Helena Kučera, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Královské Vinohrady
http://linked.open...dnocenehoVysledku	302501
http://linked.open...ai/riv/idVysledku	RIV/00064173:_____/09:00002247
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	high pressure; allergen; Api g1; celery (en)
http://linked.open.../riv/klicoveSlovo	allergen celery Api g1 high pressure
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[7E77545F6F09]
http://linked.open...i/riv/nazevZdroje	High Pressure Research
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Inactivation alergens in foods by the high pressure treatment
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Kučera, Petr Houska, Milan Vávrová, Helena
http://linked.open...ain/vavai/riv/wos	000274675700042
issn	0895-7959
number of pages	19 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/08957950903421685

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