About: Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR

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About: Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mycobacterium avium subsp. paratuberculosis (MAP) has a high degree of resistance to chemical and physical procedures frequently used for the elimination of other bacteria. Recently, a method for the determination of viability by exposure of MAP to propidium monoazide (PMA) and subsequent real time quantitative PCR (qPCR) was established and found to be comparable with culture. The aim of this study was to apply the PMA qPCR method to determine the impact of increasing concentration or time and repeated cycles of the application of selected disinfectants on MAP viability. Different MAP isolates responded to the same type of stress in different ways. The laboratory strain CAPM 6381 had the highest tolerance, while the 8819 low-passage field isolate was the most sensitive. Ultraviolet exposure caused only a partial reduction in MAP viability; all MAP isolates were relatively resistant to chlorine. Only the application of peracetic acid led to the total elimination of MAP. Repeated application of the treatments resulted in more significant decreases in MAP viability compared to single increases in the concentration or time of exposure to the disinfectant. (C) 2014 Elsevier Ltd. All rights reserved. Mycobacterium avium subsp. paratuberculosis (MAP) has a high degree of resistance to chemical and physical procedures frequently used for the elimination of other bacteria. Recently, a method for the determination of viability by exposure of MAP to propidium monoazide (PMA) and subsequent real time quantitative PCR (qPCR) was established and found to be comparable with culture. The aim of this study was to apply the PMA qPCR method to determine the impact of increasing concentration or time and repeated cycles of the application of selected disinfectants on MAP viability. Different MAP isolates responded to the same type of stress in different ways. The laboratory strain CAPM 6381 had the highest tolerance, while the 8819 low-passage field isolate was the most sensitive. Ultraviolet exposure caused only a partial reduction in MAP viability; all MAP isolates were relatively resistant to chlorine. Only the application of peracetic acid led to the total elimination of MAP. Repeated application of the treatments resulted in more significant decreases in MAP viability compared to single increases in the concentration or time of exposure to the disinfectant. (C) 2014 Elsevier Ltd. All rights reserved. (en)
Title	Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR (en)
skos:prefLabel	Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR (en)
skos:notation	RIV/00027162:_____/14:#0001128!RIV15-MZE-00027162
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LO1218), P(QH81065)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Babák, Vladimír Přibylová, Radka Králík, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	42203
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/14:#0001128
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Mycobacterium avium subsp paratuberculosis; Disinfection; Propidium monoazide quantitative PCR; Viability (en)
http://linked.open.../riv/klicoveSlovo	Disinfection Mycobacterium avium subsp paratuberculosis Viability Propidium monoazide quantitative PCR
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FFCBEFE12D36]
http://linked.open...i/riv/nazevZdroje	Veterinary journal
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination. Healthy animal as a source of wholesome food
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	201
http://linked.open...iv/tvurceVysledku	Babák, Vladimír Králík, Petr Přibylová-Dziedzinská, Radka
http://linked.open...ain/vavai/riv/wos	000342711600022
issn	1090-0233
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.tvjl.2014.05.032

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