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  • Nontuberculous mycobacteria (NTM) are ubiquitous organisms that have been isolated from a variety of environmental sources. Several NTM species are responsible for diseases in humans and/or animals known as mycobacterioses. The aim of this study was to determine the levels of NTM in the sediments and plants of five fish ponds in the Czech Republic using culture and quantitative real time PCR (qPCR). Additionally, we investigated if there was any link between environmental samples from the fish ponds and the fish occupying them. A total of 8 NTM (14.0%) were cultured from the aquatic environment. qPCR analysis showed that Mycobacterium avium hominissuis was most frequently present (54.4%), followed by Mycobacterium avium paratuberculosis (42.1%). The least frequently isolated NTM was Mycobacterium avium avium (5.3%). Thus, in this study we confirm the presence of mycobacteria in sediments and aquatic plants in fishponds, which are occupied by fish intended for human consumption. We successfully isolated NTM from the tissue of one fish and confirmed a possible transmission of mycobacteria from the aquatic environment to the fish. Consequently, the consumption of such fish represents a possible risk for consumers, particularly immunocompromised individuals. (C) 2013 Elsevier B.V. All rights reserved.
  • Nontuberculous mycobacteria (NTM) are ubiquitous organisms that have been isolated from a variety of environmental sources. Several NTM species are responsible for diseases in humans and/or animals known as mycobacterioses. The aim of this study was to determine the levels of NTM in the sediments and plants of five fish ponds in the Czech Republic using culture and quantitative real time PCR (qPCR). Additionally, we investigated if there was any link between environmental samples from the fish ponds and the fish occupying them. A total of 8 NTM (14.0%) were cultured from the aquatic environment. qPCR analysis showed that Mycobacterium avium hominissuis was most frequently present (54.4%), followed by Mycobacterium avium paratuberculosis (42.1%). The least frequently isolated NTM was Mycobacterium avium avium (5.3%). Thus, in this study we confirm the presence of mycobacteria in sediments and aquatic plants in fishponds, which are occupied by fish intended for human consumption. We successfully isolated NTM from the tissue of one fish and confirmed a possible transmission of mycobacteria from the aquatic environment to the fish. Consequently, the consumption of such fish represents a possible risk for consumers, particularly immunocompromised individuals. (C) 2013 Elsevier B.V. All rights reserved. (en)
Title
  • Analysis of sediments and plants from the system of five fishponds in the Czech Republic using culture and PCR methods
  • Analysis of sediments and plants from the system of five fishponds in the Czech Republic using culture and PCR methods (en)
skos:prefLabel
  • Analysis of sediments and plants from the system of five fishponds in the Czech Republic using culture and PCR methods
  • Analysis of sediments and plants from the system of five fishponds in the Czech Republic using culture and PCR methods (en)
skos:notation
  • RIV/00027162:_____/14:#0001105!RIV15-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0006/01/01), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika
  • FEB 15
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 2760
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/14:#0001105
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Potentially pathogenic; Zoonosis; IS900; IS901; IS1245; Food safety (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [E8A5AD8FD9F3]
http://linked.open...i/riv/nazevZdroje
  • Science of the Total Environment
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 472
http://linked.open...iv/tvurceVysledku
  • Slaná, Iva
  • Slaný, Michal
  • Klanicová, Barbora
http://linked.open...ain/vavai/riv/wos
  • 000331916100093
http://linked.open...n/vavai/riv/zamer
issn
  • 0048-9697
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.scitotenv.2013.11.132
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