About: A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum

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About: A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Mycobacterium marinum group (MMG) is a class of mycobacteria that includes M.marinum, the cause of chronic systemic infections in fish. This species occasionally causes granulomatous skin lesions in humans. Other members of MMG are mycolactone-producing mycobacteria (MPM; M.ulcerans, M.shottsii and M.pseudoshottsii). The cultivation-independent approach presented in this study brings a fast and reliable alternative to classically used cultivation methods. The developed triplex erp/IS2404 qPCR assay is based on a primary species-specific erp detection, which allows enumeration of MMG in analysed samples, and secondary IS2404 detection is suitable for the differentiation of M.marinum from MPM. The detection of M.marinum in clinical specimens and in artificially contaminated tissue samples has proven its applicability for diagnostic purposes. The Mycobacterium marinum group (MMG) is a class of mycobacteria that includes M.marinum, the cause of chronic systemic infections in fish. This species occasionally causes granulomatous skin lesions in humans. Other members of MMG are mycolactone-producing mycobacteria (MPM; M.ulcerans, M.shottsii and M.pseudoshottsii). The cultivation-independent approach presented in this study brings a fast and reliable alternative to classically used cultivation methods. The developed triplex erp/IS2404 qPCR assay is based on a primary species-specific erp detection, which allows enumeration of MMG in analysed samples, and secondary IS2404 detection is suitable for the differentiation of M.marinum from MPM. The detection of M.marinum in clinical specimens and in artificially contaminated tissue samples has proven its applicability for diagnostic purposes. (en)
Title	A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum (en)
skos:prefLabel	A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum (en)
skos:notation	RIV/00027162:_____/14:#0001096!RIV15-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QH91240), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Slaný, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	883
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/14:#0001096
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	molecular microbiology; fish mycobacteriosis; real-time PCR; bacterial typing; cutaneous infection; zoonosis (en)
http://linked.open.../riv/klicoveSlovo	zoonosis bacterial typing cutaneous infection fish mycobacteriosis real-time PCR molecular microbiology
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A236F48E8528]
http://linked.open...i/riv/nazevZdroje	Journal of Fish Diseases
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Mycobacterial infections in freshwater fish and their impact for livestock and humans.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	37
http://linked.open...iv/tvurceVysledku	Slaný, Michal
http://linked.open...ain/vavai/riv/wos	000333367800023
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0140-7775
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/jfd.12113

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