About: Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

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About: Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB) like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment. This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB) like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment. (en)
Title	Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic (en)
skos:prefLabel	Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic (en)
skos:notation	RIV/00027162:_____/14:#0001093!RIV15-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QJ1210113), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kříž, Petr Slaná, Iva Pavlík, Ivo Bartejsová, Iva Kaevska, Marija
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	31252
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/14:#0001093
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SLAUGHTERED DOMESTIC PIGS; POSTMORTEM INSPECTION PROCEDURES; RHODOCOCCUS-EQUI; CAUSAL FACTORS; LESIONS; COMPLEX; HOMINISSUIS; SAMPLES; SWINE; PCR (en)
http://linked.open.../riv/klicoveSlovo	HOMINISSUIS PCR LESIONS CAUSAL FACTORS SAMPLES COMPLEX POSTMORTEM INSPECTION PROCEDURES RHODOCOCCUS-EQUI SLAUGHTERED DOMESTIC PIGS SWINE
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D85822E2326B]
http://linked.open...i/riv/nazevZdroje	Journal of Food Protection
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine The effect of processing meat from farm and wild animals in traditional and non-traditional ways on the occurrence of emerging foodborne viral, bacterial and parasitic agents in the final products.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Kříž, Petr Kaevska, Marija Pavlík, Ivo Slaná, Iva Bartejsová, Iva
http://linked.open...ain/vavai/riv/wos	000329593300020
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0362-028X
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.4315/0362-028X.JFP-13-036

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