About: DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa

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About: DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by Sperm chromatin structure assay. In the first experiment, chromatin integrity of fresh semen from 60 dogs with different history of fertility was compared with other sperm parameters. In the second experiment, chromatin status was measured in fresh and chilled spermatozoa. In the third experiment, the chromatin status was measured in fresh and cryopreserved spermatozoa. Concerning a potential cause of reduced male fertility, the assessment of chromatin integrity in fresh dog ejaculates failed to bring any additional information to the results obtained by other techniques of semen analysis. It follows from the current study that neither a 10-day preservation of canine sperm chilled in commercial extenders nor a long-term cryopreservation in the extenders recommended for canine sperm preservation produced adverse effects on sperm chromatin integrity. Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by Sperm chromatin structure assay. In the first experiment, chromatin integrity of fresh semen from 60 dogs with different history of fertility was compared with other sperm parameters. In the second experiment, chromatin status was measured in fresh and chilled spermatozoa. In the third experiment, the chromatin status was measured in fresh and cryopreserved spermatozoa. Concerning a potential cause of reduced male fertility, the assessment of chromatin integrity in fresh dog ejaculates failed to bring any additional information to the results obtained by other techniques of semen analysis. It follows from the current study that neither a 10-day preservation of canine sperm chilled in commercial extenders nor a long-term cryopreservation in the extenders recommended for canine sperm preservation produced adverse effects on sperm chromatin integrity. (en)
Title	DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa (en)
skos:prefLabel	DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa (en)
skos:notation	RIV/00027162:_____/12:#0000872!RIV13-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP523/08/P561), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Rybář, Roman Přinosilová, Petra Hlavicová, Jaroslava Zajícová, Atanáska
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	131762
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/12:#0000872
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	dog; DNA fragmentation index; sperm chromatin structure assay (SCSA), cryopreservation; chilled semen (en)
http://linked.open.../riv/klicoveSlovo	cryopreservation dog DNA fragmentation index chilled semen sperm chromatin structure assay (SCSA)
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[C8533A8BA374]
http://linked.open...i/riv/nazevZdroje	Veterinární Medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of dog sperm cell resistance to cold stress
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Přinosilová, Petra Rybář, Roman Hlavicová, Jaroslava Zajícová, Atanáska
http://linked.open...ain/vavai/riv/wos	000303100100006
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0375-8427
number of pages	10 (xsd:int)

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