About: Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples

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About: Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to optimise a two-tube reverse transcription triplex quantitative real time PCR (qRT-PCR) combining amplification of two loci with an internal amplification control (IAC) for detection and quantitation of hepatitis E virus (HEV) RNA and to validate its performance on a pool of biological samples. Optimisation was performed on serially diluted %22home-made%22 RNA standards. The limit of detection was determined experimentally as 10 copies/mu l of the RNA standard for both amplification targets. The qRT-PCR was validated on a cohort of samples originating from 48 wild boars (Sus scrofa), 17 fallow deer (Dama dama) and 28 mouflons (Ovis musimon), with nested RT-PCR used as a reference method. qRT-PCR was found to be more specific for the detection of HEV RNA in examined samples. HEV RNA was detected in samples of five more animals (one wild boar and four mouflons) in comparison with nested RT-PCR. (C) 2011 Elsevier B.V. All rights reserved. The aim of this study was to optimise a two-tube reverse transcription triplex quantitative real time PCR (qRT-PCR) combining amplification of two loci with an internal amplification control (IAC) for detection and quantitation of hepatitis E virus (HEV) RNA and to validate its performance on a pool of biological samples. Optimisation was performed on serially diluted %22home-made%22 RNA standards. The limit of detection was determined experimentally as 10 copies/mu l of the RNA standard for both amplification targets. The qRT-PCR was validated on a cohort of samples originating from 48 wild boars (Sus scrofa), 17 fallow deer (Dama dama) and 28 mouflons (Ovis musimon), with nested RT-PCR used as a reference method. qRT-PCR was found to be more specific for the detection of HEV RNA in examined samples. HEV RNA was detected in samples of five more animals (one wild boar and four mouflons) in comparison with nested RT-PCR. (C) 2011 Elsevier B.V. All rights reserved. (en)
Title	Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples (en)
skos:prefLabel	Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples (en)
skos:notation	RIV/00027162:_____/12:#0000871!RIV13-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(OC08045), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Slaná, Iva Králík, Petr Pavlík, Ivo Vašíčková, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	156910
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/12:#0000871
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Internal amplification control; RNA quantitation; Zoonosis (en)
http://linked.open.../riv/klicoveSlovo	Zoonosis RNA quantitation Internal amplification control
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[10D32540937C]
http://linked.open...i/riv/nazevZdroje	Journal of Virological Methods
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Hepatitis E virus detection in market pigs, in the processing plants and pork-based foodstuffs in the trade network of the Czech Republic.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	180
http://linked.open...iv/tvurceVysledku	Králík, Petr Pavlík, Ivo Slaná, Iva Vašíčková, Petra
http://linked.open...ain/vavai/riv/wos	000301884200007
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0166-0934
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.jviromet.2011.12.007

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