About: Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

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About: Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis (with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and delta SPI1-5 mutant) with subpopulations of porcine white blood cells (WBC). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. Delta SPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wildtype S. Enteritidis did. Electron microscopy showed that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not develop into a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes. We were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis (with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and delta SPI1-5 mutant) with subpopulations of porcine white blood cells (WBC). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. Delta SPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wildtype S. Enteritidis did. Electron microscopy showed that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not develop into a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes. (en)
Title	Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes (en)
skos:prefLabel	Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes (en)
skos:notation	RIV/00027162:_____/11:#0000802!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(GA524/08/1606), P(QH81062), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Malcová, Marcela Faldyna, Martin Štěpánová, Hana Matiašovic, Ján Volf, Jiří Rychlík, Ivan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	187326
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000802
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	neutrophil; macrophage; lymphocyte; pig; flow cytometry; SCV development (en)
http://linked.open.../riv/klicoveSlovo	SCV development lymphocyte macrophage neutrophil flow cytometry pig
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0EFB1D8EFD48]
http://linked.open...i/riv/nazevZdroje	FEMS MICROBIOLOGY LETTERS
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Cellular immune response to defined deleted mutants of Salmonella in pigs Immunology and immunodiagnostics of salmonella infection in pig Center for Advanced Microbiology & Immunology Research in Veterinary Medicine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	321
http://linked.open...iv/tvurceVysledku	Faldyna, Martin Malcová, Marcela Matiašovic, Ján Rychlík, Ivan Volf, Jiří Štěpánová, Hana
http://linked.open...ain/vavai/riv/wos	000292337200006
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0378-1097
number of pages	6 (xsd:int)

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