AttributesValues
rdf:type
Description
  • Products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected cattle were examined for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Viable MAP cells were detected using culture in fermentors for up to 2 months; the presence of MAP DNA was demonstrated in all anaerobic fermentors and digestate up to 16 months after initiation of work at a biogas plant, using IS900 real time PCR (qPCR). According to these results, a fermentation process that extended beyond 2 months removed all viable MAP cells and therefore rendered its product MAP free. MAP DNA was found during all the examined periods, which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined.
  • Products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected cattle were examined for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Viable MAP cells were detected using culture in fermentors for up to 2 months; the presence of MAP DNA was demonstrated in all anaerobic fermentors and digestate up to 16 months after initiation of work at a biogas plant, using IS900 real time PCR (qPCR). According to these results, a fermentation process that extended beyond 2 months removed all viable MAP cells and therefore rendered its product MAP free. MAP DNA was found during all the examined periods, which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined. (en)
Title
  • Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle
  • Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle (en)
skos:prefLabel
  • Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle
  • Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle (en)
skos:notation
  • RIV/00027162:_____/11:#0000761!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 219988
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/11:#0000761
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ANAEROBIC-DIGESTION; PATHOGENIC BACTERIA; JOHNES-DISEASE; SURVIVAL; ENVIRONMENT; CULTURE; SLURRY; PREVALENCE; TISSUES; STORAGE (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [0E14DA709D16]
http://linked.open...i/riv/nazevZdroje
  • APPLIED AND ENVIRONMENTAL MICROBIOLOGY
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 77
http://linked.open...iv/tvurceVysledku
  • Králová, Alena
  • Pavlík, Ivo
  • Přibylová, Radka
  • Slaná, Iva
http://linked.open...ain/vavai/riv/wos
  • 000289773100036
http://linked.open...n/vavai/riv/zamer
issn
  • 0099-2240
number of pages
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