About: Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products

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About: Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this work was to examine various purchased meat products and to find out if any traces of Mycobacterium avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis could be detected in these samples. Analysis of the meat products (raw, cooked, and fermented) was performed using a real-time quantitative PCR (qPCR) method for the detection of specific insertion sequences. Of the 77 meat samples, 17 (22%) were found to contain M. avium subsp. paratuberculosis DNA, 4 (5%) samples contained Mycobacterium avium subsp. avium DNA, and in 12 (16%) samples M. avium subsp. hominissuis DNA was detected. The concentration of M. avium subsp. paratuberculosis and M. avium subsp. hominissuis DNA in some meat products exceeded 104 genomes per g. Culture examination of these mycobacterial subspecies was negative. By analyzing a range of meat products, we have provided evidence to support the hypothesis that M. avium is present in everyday commodities sold to the general public. The aim of this work was to examine various purchased meat products and to find out if any traces of Mycobacterium avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis could be detected in these samples. Analysis of the meat products (raw, cooked, and fermented) was performed using a real-time quantitative PCR (qPCR) method for the detection of specific insertion sequences. Of the 77 meat samples, 17 (22%) were found to contain M. avium subsp. paratuberculosis DNA, 4 (5%) samples contained Mycobacterium avium subsp. avium DNA, and in 12 (16%) samples M. avium subsp. hominissuis DNA was detected. The concentration of M. avium subsp. paratuberculosis and M. avium subsp. hominissuis DNA in some meat products exceeded 104 genomes per g. Culture examination of these mycobacterial subspecies was negative. By analyzing a range of meat products, we have provided evidence to support the hypothesis that M. avium is present in everyday commodities sold to the general public. (en)
Title	Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products (en)
skos:prefLabel	Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products (en)
skos:notation	RIV/00027162:_____/11:#0000758!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Slaná, Iva Pavlík, Ivo Vondrušková, Hana Klanicová, Barbora Kaevska, Marija
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	225865
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000758
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Meat products; Mycobacterium avium; detection (en)
http://linked.open.../riv/klicoveSlovo	Mycobacterium avium detection Meat products
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[EC5C4B2EE040]
http://linked.open...i/riv/nazevZdroje	Journal of Food Protection
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	74
http://linked.open...iv/tvurceVysledku	Kaevska, Marija Pavlík, Ivo Slaná, Iva Vondrušková, Hana Klanicová, Barbora
http://linked.open...ain/vavai/riv/wos	000289700800018
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0362-028X
number of pages	5 (xsd:int)

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