About: Differentiation of Staphylococcus spp. by high-resolution melting analysis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	High resolution melting analysis (HRMA) is a fast post-PCR method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range 16 rRNA PCR. Genomic DNA samples isolated from 12 reference staphylococcal strains were subjected to a HRMA in the presence of fluorescent dye EvaGreen. Melting profiles were used as molecular fingerprints for bacterial species differentiation. S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unrelated strain. High resolution melting analysis (HRMA) is a fast post-PCR method to scan for sequence variations in a target gene. The aim of this study was to test the potential of HRMA to distinguish particular bacterial species of the Staphylococcus genus even when using a broad-range 16 rRNA PCR. Genomic DNA samples isolated from 12 reference staphylococcal strains were subjected to a HRMA in the presence of fluorescent dye EvaGreen. Melting profiles were used as molecular fingerprints for bacterial species differentiation. S. saprophyticus and S. xylosus resulted in undistinguishable profiles because of their identical sequences in the analyzed 16S rRNA region. The remaining reference strains were fully differentiated either directly or via high-resolution plots obtained by heteroduplex formation between coamplified PCR products of the tested staphylococcal strain and phylogenetically unrelated strain. (en)
Title	Differentiation of Staphylococcus spp. by high-resolution melting analysis Differentiation of Staphylococcus spp. by high-resolution melting analysis (en)
skos:prefLabel	Differentiation of Staphylococcus spp. by high-resolution melting analysis Differentiation of Staphylococcus spp. by high-resolution melting analysis (en)
skos:notation	RIV/00027162:_____/11:#0000749!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita	S V Z
http://linked.open...avai/riv/aktivity	S, V, Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Slaný, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	254389
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000749
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HRMA; real-time PCR; 16S rRNA; bacterial detection (en)
http://linked.open.../riv/klicoveSlovo	HRMA 16S rRNA real-time PCR bacterial detection
http://linked.open...odStatuVydavatele	CA - Kanada
http://linked.open...ontrolniKodProRIV	[26771DF97272]
http://linked.open...i/riv/nazevZdroje	Canadian Journal of Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Slaný, Michal Freiberger, T. Růžička, F. Němcová, E. Vanerková, M. Zaloudíková, B.
http://linked.open...ain/vavai/riv/wos	000285556000008
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0008-4166
number of pages	10 (xsd:int)

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