About: Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAP DNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNA isolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the culture. This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAP DNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNA isolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the culture. (en)
Title	Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR (en)
skos:prefLabel	Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR (en)
skos:notation	RIV/00027162:_____/11:#0000743!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Babák, Vladimír Slaná, Iva Králík, Petr Králová, Alena Pavlík, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	194022
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000743
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MAP; Johne's disease; Faeces DNA isolation; Paratuberculosis; qPCR (en)
http://linked.open.../riv/klicoveSlovo	Johne's disease qPCR Faeces DNA isolation MAP Paratuberculosis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[FA4E33EB21E6]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	149
http://linked.open...iv/tvurceVysledku	Babák, Vladimír Králová, Alena Králík, Petr Pavlík, Ivo Slaná, Iva Whitlock, R. H.
http://linked.open...ain/vavai/riv/wos	000289031600016
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0378-1135
number of pages	6 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 24 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software