About: Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription     Goto   Sponge   NotDistinct   Permalink

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  • Unlike all of the other retrons, the bacterial retron reverse transcriptase RrtE is capable of synthesizing small double-stranded DNA (sdsDNA) from template RNA. In this study, we analyzed the biosynthesis of the sdsDNA by RrtE in detail. We found out that the initiation of reverse transcription was dependent on a novel self-priming mechanism utilizing a free 3'OH of RNA that is reverse-transcribed into sdsDNA. The priming of the sdsDNA synthesis was not dependent on any particular nucleotide being used as a donor of 3'OH (unlike all of the other retrons, which prime from 2'OH of a particular guanosine) or any particular nucleotide being introduced into the sdsDNA first. Due to the relaxed demands for the initiation of reverse transcription, RrtE has the potential to generate dsDNA from different RNA transcripts in vivo.
  • Unlike all of the other retrons, the bacterial retron reverse transcriptase RrtE is capable of synthesizing small double-stranded DNA (sdsDNA) from template RNA. In this study, we analyzed the biosynthesis of the sdsDNA by RrtE in detail. We found out that the initiation of reverse transcription was dependent on a novel self-priming mechanism utilizing a free 3'OH of RNA that is reverse-transcribed into sdsDNA. The priming of the sdsDNA synthesis was not dependent on any particular nucleotide being used as a donor of 3'OH (unlike all of the other retrons, which prime from 2'OH of a particular guanosine) or any particular nucleotide being introduced into the sdsDNA first. Due to the relaxed demands for the initiation of reverse transcription, RrtE has the potential to generate dsDNA from different RNA transcripts in vivo. (en)
Title
  • Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription
  • Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription (en)
skos:prefLabel
  • Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription
  • Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription (en)
skos:notation
  • RIV/00027162:_____/11:#0000736!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0006/01/01), P(LC06030), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika
  • 21
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 226879
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/11:#0000736
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Retron; Self-priming mechanism; Reverse transcriptase; Salmonella (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV
  • [CA0EB3BD4284]
http://linked.open...i/riv/nazevZdroje
  • Cellular and Molecular Life Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 68
http://linked.open...iv/tvurceVysledku
  • Pilousová, Jitka
  • Rychlík, Ivan
http://linked.open...ain/vavai/riv/wos
  • 000296469200013
http://linked.open...n/vavai/riv/zamer
issn
  • 1420-682X
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s00018-011-0671-0
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