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rdf:type
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Description
| - Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserved.
- Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserved. (en)
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Title
| - Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces
- Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces (en)
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skos:prefLabel
| - Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces
- Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces (en)
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skos:notation
| - RIV/00027162:_____/11:#0000731!RIV12-MZE-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(QF4051), Z(MZE0002716201)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/11:#0000731
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Rotavirus groups A-C; Blocking ELISA; Monoclonal antibody; PCR; Viral gastroenteritis; Swine (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Nevoránková, Zora
- Pšikal, Ivan
- Rodák, Ladislav
- Smítalová, Radka
- Valíček, Lubomír
- Šmíd, Bedřich
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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