About: Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

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About: Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserved. Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserved. (en)
Title	Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces (en)
skos:prefLabel	Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces (en)
skos:notation	RIV/00027162:_____/11:#0000731!RIV12-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QF4051), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Valíček, Lubomír Pšikal, Ivan Nevoránková, Zora Smítalová, Radka Rodák, Ladislav Šmíd, Bedřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	191044
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000731
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Rotavirus groups A-C; Blocking ELISA; Monoclonal antibody; PCR; Viral gastroenteritis; Swine (en)
http://linked.open.../riv/klicoveSlovo	Blocking ELISA Monoclonal antibody PCR Rotavirus groups A-C Swine Viral gastroenteritis
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[8A7DA2736200]
http://linked.open...i/riv/nazevZdroje	Veterinary Journal
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of biotechnological procedures in the diagnosis and immunoprophylaxis of viral gastroenteritides of swine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	187
http://linked.open...iv/tvurceVysledku	Nevoránková, Zora Pšikal, Ivan Rodák, Ladislav Smítalová, Radka Valíček, Lubomír Šmíd, Bedřich
http://linked.open...ain/vavai/riv/wos	000288520000029
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	1090-0233
number of pages	3 (xsd:int)

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