About: Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was two-year monitoring of one mouflon farmed herd for the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using IS900 real-time PCR method. This study followed the previous monitoring of the herd using cultivation method which showed only minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900 positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the IS900 sequence was determined in 2008 as well as in 2009. Almost one third of mouflon lambs born between the first and second sampling (30.4%) were proven to have the MAP sequence in their faeces. Screening of mouflons' farm showed 75% positivity of the environmental samples. Infected animals without clinical symptoms and not revealed using low sensitivity cultivation present a source of infection for other animals. Therefore, real-time PCR has unambiguous role in paratuberculosis control programs. The aim of this study was two-year monitoring of one mouflon farmed herd for the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using IS900 real-time PCR method. This study followed the previous monitoring of the herd using cultivation method which showed only minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900 positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the IS900 sequence was determined in 2008 as well as in 2009. Almost one third of mouflon lambs born between the first and second sampling (30.4%) were proven to have the MAP sequence in their faeces. Screening of mouflons' farm showed 75% positivity of the environmental samples. Infected animals without clinical symptoms and not revealed using low sensitivity cultivation present a source of infection for other animals. Therefore, real-time PCR has unambiguous role in paratuberculosis control programs. (en)
Title	Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report (en)
skos:prefLabel	Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report (en)
skos:notation	RIV/00027162:_____/10:#0000699!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Babák, Vladimír Slaná, Iva Přibylová, Radka Pavlík, Ivo Hruška, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	273322
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000699
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MAP; Johne's disease; faeces; environment; qPCR (en)
http://linked.open.../riv/klicoveSlovo	Johne's disease environment qPCR MAP faeces
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[CFAC8270FCE9]
http://linked.open...i/riv/nazevZdroje	Veterinární Medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Babák, Vladimír Hruška, Karel Pavlík, Ivo Přibylová, Radka Slaná, Iva Lamka, J.
http://linked.open...ain/vavai/riv/wos	000285647200007
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0375-8427
number of pages	6 (xsd:int)

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