About: Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations

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About: Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands. The aim of the present study was to study by culture, and a quantitative real-time polymerase chain reaction, the presence of bacteria in consecutive semen, blood, and fecal samples collected from one infected Piedmont breeding bull during a 380 day period. Five out of seven blood samples and all nine semen samples were positive on real-time quantitative polymerase chain reaction with 101 to 102 and 102 to 104 copies of IS900/F57 per ml, respectively. In all, there were 9 fecal culture positive samples with too numerous to count colony forming units and positive real-time quantitiative polymerase chain reaction ranging from 105 to 107 copies of IS900/F57. After the bull was euthanized, Mycobacterium avium subsp. paratuberculosis was cultured from various parts of small and large intestines, liver tissue, lymph nodes, and from epididymis and vesicular glands. (en)
Title	Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations (en)
skos:prefLabel	Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations Consecutive Excretion of Mycobacterium avium Subspecies paratuberculosis in Semen of a Breeding Bull Compared to the Distribution in Feces, Tissue and Blood by IS900 and F57 Quantitative Real-Time PCR and Culture Examinations (en)
skos:notation	RIV/00027162:_____/10:#0000695!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Slaná, Iva Králík, Petr Pavlík, Ivo Beran, Vladimír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	251871
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000695
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Breeding bull; Cattle; Johne's disease; paratuberculosis; semen analysis (en)
http://linked.open.../riv/klicoveSlovo	Cattle Johne's disease paratuberculosis semen analysis Breeding bull
http://linked.open...odStatuVydavatele	JP - Japonsko
http://linked.open...ontrolniKodProRIV	[C72BD71CEDB8]
http://linked.open...i/riv/nazevZdroje	Journal of Veterinary Medical Science
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	72
http://linked.open...iv/tvurceVysledku	Králík, Petr Pavlík, Ivo Slaná, Iva Beran, Vladimír Aurich, C. Baumgartner, W. Khol, J. L.
http://linked.open...ain/vavai/riv/wos	000284246900005
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0916-7250
number of pages	6 (xsd:int)

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