About: Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes

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About: Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We assessed: the change in nitric oxide synthase (NOS) isoforms' expression and intracellular localization during meiotic maturation; and the effect of NOS inhibition by L-NAME and aminoguanidine (AG) on meiotic maturation. All three NOS isoforms (eNOS, iNOS and nNOS) and NOS mRNA were found in both porcine oocytes and their cumulus cells except for nNOS mRNA, which was not detected in the cumulus cells. NOS isoforms differed in their intracellular localization in the oocyte: while iNOS protein was dispersed in cytoplasm, nNOS was localized in cytoplasm and in GV and eNOS was present in dots in the cytoplasm, GV and was associated with spindles. L-NAME inhibitor suppressed MI to MII transition and at the highest concentration also affected GVBD; in contrast also AG inhibited primarily GVBD. AG inhibited GVBD in DO, but L-NAME had no significant effect on the GVBD of these cells. During meiotic maturation, NO is formed in COC and the NO formed by cumulus cells is necessary for the process of GVBD. We assessed: the change in nitric oxide synthase (NOS) isoforms' expression and intracellular localization during meiotic maturation; and the effect of NOS inhibition by L-NAME and aminoguanidine (AG) on meiotic maturation. All three NOS isoforms (eNOS, iNOS and nNOS) and NOS mRNA were found in both porcine oocytes and their cumulus cells except for nNOS mRNA, which was not detected in the cumulus cells. NOS isoforms differed in their intracellular localization in the oocyte: while iNOS protein was dispersed in cytoplasm, nNOS was localized in cytoplasm and in GV and eNOS was present in dots in the cytoplasm, GV and was associated with spindles. L-NAME inhibitor suppressed MI to MII transition and at the highest concentration also affected GVBD; in contrast also AG inhibited primarily GVBD. AG inhibited GVBD in DO, but L-NAME had no significant effect on the GVBD of these cells. During meiotic maturation, NO is formed in COC and the NO formed by cumulus cells is necessary for the process of GVBD. (en)
Title	Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes (en)
skos:prefLabel	Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes (en)
skos:notation	RIV/00027162:_____/10:#0000685!RIV11-GA0-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD523/08/H064), P(QG50052), Z(MSM6046070901), Z(MSM6046137305), Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ješeta, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	274897
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000685
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cumulus cells; Meiotic maturation; NOS; Oocyte; Pig (en)
http://linked.open.../riv/klicoveSlovo	Meiotic maturation Oocyte Pig Cumulus cells NOS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[19BA7637C7D5]
http://linked.open...i/riv/nazevZdroje	Zygote
http://linked.open...in/vavai/riv/obor	GG
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Reproductive biotechniques in pigs Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Kott, T. Ješeta, Michal Tůmová, L. Sedmíková, M. Lipovová, P. Petr, J. Jílek, F. Chmelíková, E.
http://linked.open...ain/vavai/riv/wos	000280163300007
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje. Teoretické základy potravinářských a biochemických technologií Od rostoucího oocytu k produkci embryí in vitro a embryonálním kmenovým buňkám: Vývojové modely a strategie produkce zárodečných, embryonálních a somatických buněk in vitro v kontextu s jejich využitím v programu zachování genetických živočišných zdrojů
issn	0967-1994
number of pages	10 (xsd:int)

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