About: Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA

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About: Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to determine whether Salmonella Typhimurium resides predominantly intra- or extracellularly in tonsils of pigs and to examine the contribution of biofilm-associated genes csgA, csgD and adrA. Single cell suspensions were prepared from tonsils of orally inoculated pigs to determine the ratio of extracellular versus intracellular bacteria. Both at 5 and 28 days post-inoculation (pi), the majority of Salmonella bacteria was found extracellularly in porcine tonsils. To determine the contribution of biofilm formation in extracellular persistence, pigs were orally inoculated with a mixture of Salmonella Typhimurium wild type strain and csgA, csgD or adrA mutants. At 10 days pi, equal numbers of both wild type and mutant Salmonella bacteria were found not only in tonsils, but also in ileum, ileum contents, ileocecal lymph nodes and faeces. In conclusion, we showed that Salmonella Typhimurium resides extracellularly in porcine tonsils, using a biofilm independent mechanism. The aim of this study was to determine whether Salmonella Typhimurium resides predominantly intra- or extracellularly in tonsils of pigs and to examine the contribution of biofilm-associated genes csgA, csgD and adrA. Single cell suspensions were prepared from tonsils of orally inoculated pigs to determine the ratio of extracellular versus intracellular bacteria. Both at 5 and 28 days post-inoculation (pi), the majority of Salmonella bacteria was found extracellularly in porcine tonsils. To determine the contribution of biofilm formation in extracellular persistence, pigs were orally inoculated with a mixture of Salmonella Typhimurium wild type strain and csgA, csgD or adrA mutants. At 10 days pi, equal numbers of both wild type and mutant Salmonella bacteria were found not only in tonsils, but also in ileum, ileum contents, ileocecal lymph nodes and faeces. In conclusion, we showed that Salmonella Typhimurium resides extracellularly in porcine tonsils, using a biofilm independent mechanism. (en)
Title	Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA (en)
skos:prefLabel	Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA (en)
skos:notation	RIV/00027162:_____/10:#0000671!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	N
http://linked.open...avai/riv/aktivity	N
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Volf, Jiří Rychlík, Ivan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	286294
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000671
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Salmonella; tonsil; pig; virulence (en)
http://linked.open.../riv/klicoveSlovo	Salmonella tonsil virulence pig
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E86DCC01AD46]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	144
http://linked.open...iv/tvurceVysledku	Rychlík, Ivan Volf, Jiří Haesebrouck, F. Pasmans, F. Boyen, F. Leyman, B. Van Parys, A. Verbrugghe, E.
http://linked.open...ain/vavai/riv/wos	000279532600012
issn	0378-1135
number of pages	7 (xsd:int)

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