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Description
| - In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content.
- In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content. (en)
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Title
| - Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate
- Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
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skos:prefLabel
| - Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate
- Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
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skos:notation
| - RIV/00027162:_____/10:#0000657!RIV11-AV0-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(KAN200520703), Z(MZE0002716202)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/10:#0000657
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - alpha-tocopheryl succinate; liposomes; stability; lyophilisation; high-performance liquid chromatography (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Koudelka, Štěpán
- Mašek, Josef
- Turánek, Jaroslav
- Turánek-Knötigová, Pavlína
- Neužil, J.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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