About: Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR

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About: Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs (ten were infected with MAA, ten with MAH and two negative controls). From each animal, 21 different tissue samples and blood were tested by microscopy, culture and qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health. Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs (ten were infected with MAA, ten with MAH and two negative controls). From each animal, 21 different tissue samples and blood were tested by microscopy, culture and qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health. (en)
Title	Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR (en)
skos:prefLabel	Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR (en)
skos:notation	RIV/00027162:_____/10:#0000638!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1B53009), P(ED0006/01/01), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Slaná, Iva Králík, Petr Pavlík, Ivo Kaevska, Marija
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	254742
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000638
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Mycobacterium; Competitive PCR; Avian tuberculosis; Internal amplification control; Food safety; Zoonosis (en)
http://linked.open.../riv/klicoveSlovo	Mycobacterium Zoonosis Food safety Internal amplification control Avian tuberculosis Competitive PCR
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[1C3B55D2D113]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Avian tuberculosis and mycobacterioses: the development of methods for theirs prevention and eradication by study of mycobacterial sources and pathogenesis of infection
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	144
http://linked.open...iv/tvurceVysledku	Králík, Petr Kaevska, Marija Pavlík, Ivo Slaná, Iva Horváthová, A.
http://linked.open...ain/vavai/riv/wos	000281358600026
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0378-1135
number of pages	7 (xsd:int)

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