About: Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment

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About: Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells. We have evaluated a promising technology combining sample treatment with propidium monoazide (PMA) prior to DNA extraction for selective detection of cells with intact cell membranes with detection of sequence element F57 by quantitative PCR (F57 qPCR). Element F57 is unique for MAP and is not known to exist in any other bacterial species. Conditions of PMA treatment were optimised for MAP isolate 7082 using live and heat-killed cells and comparing different DNA extraction procedures. The subsequent successful application of the optimised protocol to four other MAP isolates of different origins suggested that the optimised protocol might be broadly applicable to different MAP strains. Furthermore, different equations were compared to use the data resulting from this technology to optimally predict the percentage of live MAP cells in mixtures containing both live and dead cells. (en)
Title	Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment (en)
skos:prefLabel	Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment Mycobacterium avium subsp paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment (en)
skos:notation	RIV/00027162:_____/10:#0000633!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	Suppl 1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Králík, Petr Pavlík, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	273323
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000633
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MAP; Johne's disease; PMA; Paratuberculosis; Crohn's disease (en)
http://linked.open.../riv/klicoveSlovo	Johne's disease MAP Paratuberculosis Crohn's disease PMA
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[81788D5FA23C]
http://linked.open...i/riv/nazevZdroje	International Journal of Food Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	141
http://linked.open...iv/tvurceVysledku	Králík, Petr Pavlík, Ivo Nocker, A.
http://linked.open...ain/vavai/riv/wos	000281830700010
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0168-1605
number of pages	7 (xsd:int)

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