About: Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study three different methods (quantitative real time PCR, combined phage IS900 PCR and conventional cultivation) were used to detect the presence of MAP in bulk tank milk (BTM) and cheese originating from sheep, goat and mixed milks from farms and products in Cyprus. During the first survey the presence of MAP was detected in 63 (28.6%) of cows' BTM samples by quantitative real time PCR. A second survey of BTM used a new combined phage IS900 PCR assay, and in this case MAP was detected in 50 (22.2%) samples showing a good level of agreement by both methods. The two isolates recovered from BTM were identified by IS1311 PCR REA as cattle and sheep strains, respectively. In contrast when cheese samples were tested, MAP DNA was detected by quantitative real time PCR in seven (25.0%) samples (n=28). However no viable MAP was detected when either the combined phage IS900 PCR or conventional culture methods were used. In this study three different methods (quantitative real time PCR, combined phage IS900 PCR and conventional cultivation) were used to detect the presence of MAP in bulk tank milk (BTM) and cheese originating from sheep, goat and mixed milks from farms and products in Cyprus. During the first survey the presence of MAP was detected in 63 (28.6%) of cows' BTM samples by quantitative real time PCR. A second survey of BTM used a new combined phage IS900 PCR assay, and in this case MAP was detected in 50 (22.2%) samples showing a good level of agreement by both methods. The two isolates recovered from BTM were identified by IS1311 PCR REA as cattle and sheep strains, respectively. In contrast when cheese samples were tested, MAP DNA was detected by quantitative real time PCR in seven (25.0%) samples (n=28). However no viable MAP was detected when either the combined phage IS900 PCR or conventional culture methods were used. (en)
Title	Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese (en)
skos:prefLabel	Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese Rapid detection methods for viable Mycobacterium avium subspecies paratuberculosis in milk and cheese (en)
skos:notation	RIV/00027162:_____/10:#0000630!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	Suppl. 1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Slaná, Iva Pavlík, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	283913
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000630
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	milk; culture; PCR; decontamination; food safety; real time PCR (en)
http://linked.open.../riv/klicoveSlovo	food safety PCR culture decontamination milk real time PCR
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C3F0B68CD253]
http://linked.open...i/riv/nazevZdroje	International Journal of Food Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	141
http://linked.open...iv/tvurceVysledku	Pavlík, Ivo Slaná, Iva Botsaris, G. Dodd, C. Economides, C. Liapi, M. Rees, C.
http://linked.open...ain/vavai/riv/wos	000281830700011
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0168-1605
number of pages	4 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 77 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software