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rdf:type
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Description
| - The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production.
- The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production. (en)
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Title
| - Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
- Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization (en)
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skos:prefLabel
| - Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
- Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization (en)
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skos:notation
| - RIV/00027162:_____/10:#0000619!RIV11-GA0-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GD523/08/H064), P(QI101A166), Z(MZE0002716202)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/10:#0000619
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - cryopreserved spermatozoa; AR progress; porcine IVF (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hulínská, Pavlína
- Ješeta, Michal
- Machatková, Marie
- Martečíková, Soňa
- Rečková, Zuzana
- Pavlík, A.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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