About: Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to assess functional status of spermatozoa after cryopreservation, separation and capacitation to characterize the influence of these procedures on spermatozoa functional parameters. Boar semen were frozen-thawed, motile spermatozoa were separated on Percoll-gradient and capacitated by 1mM caffeine. Motility, viability and chromatine integrity of spermatozoa were evaluated by phase contrast, Annexin V-FICT apoptosis kit and SCSA assay respectively. The high cryotolerant- (HCT) and the low cryotolerant- (LCT) boars were classified in previous experiments. There were no differences in motility and viability spermatozoa between both boar groups before freezing but after thawing, the significantly higher motility and viability were found for HCT- compared with those for LCT-boars. Separation was more effective in the LCT- than in HCT- boars.. In both groups, DNA of spermatozoa was highly stable during cryopreservation, separation and capacitation. The aim of this study was to assess functional status of spermatozoa after cryopreservation, separation and capacitation to characterize the influence of these procedures on spermatozoa functional parameters. Boar semen were frozen-thawed, motile spermatozoa were separated on Percoll-gradient and capacitated by 1mM caffeine. Motility, viability and chromatine integrity of spermatozoa were evaluated by phase contrast, Annexin V-FICT apoptosis kit and SCSA assay respectively. The high cryotolerant- (HCT) and the low cryotolerant- (LCT) boars were classified in previous experiments. There were no differences in motility and viability spermatozoa between both boar groups before freezing but after thawing, the significantly higher motility and viability were found for HCT- compared with those for LCT-boars. Separation was more effective in the LCT- than in HCT- boars.. In both groups, DNA of spermatozoa was highly stable during cryopreservation, separation and capacitation. (en)
Title	Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro (en)
skos:prefLabel	Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro (en)
skos:notation	RIV/00027162:_____/09:#0000566!RIV10-MZE-00027162
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GD523/08/H064), P(QG50052)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Machatková, Marie Ješeta, Michal Hulínská, Pavlína Martečíková, Soňa Rybář, Roman
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	306653
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/09:#0000566
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Boar; semen; cryopreservation; spermatozoa assessment; annexin-V; SCSA (en)
http://linked.open.../riv/klicoveSlovo	semen Boar cryopreservation SCSA annexin-V spermatozoa assessment
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[D75DAAD611E0]
http://linked.open...i/riv/nazevZdroje	Research in Pig Breeding
http://linked.open...in/vavai/riv/obor	GI
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Reproductive biotechniques in pigs Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	3
http://linked.open...iv/tvurceVysledku	Rybář, Roman Hulínská, Pavlína Ješeta, Michal Machatková, Marie Martečíková, Soňa Pavlík, Aleš
issn	1802-7547
number of pages	5 (xsd:int)

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