About: Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea

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About: Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1. The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1. (en)
Title	Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea (en)
skos:prefLabel	Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea (en)
skos:notation	RIV/00027162:_____/09:#0000496!RIV10-MZE-00027162
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Dvořáková, Hana Valíček, Lubomír Prodělalová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	316222
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/09:#0000496
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Teschovirus; PTV-1; fecal samples; reverse transcription; polymerase chain reaction (en)
http://linked.open.../riv/klicoveSlovo	PTV-1 polymerase chain reaction fecal samples Teschovirus reverse transcription
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[7A6054E89311]
http://linked.open...i/riv/nazevZdroje	Veterinární medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Valíček, Lubomír Malenovská, Hana Prodělalová, Jana
http://linked.open...ain/vavai/riv/wos	000272392800002
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0375-8427
number of pages	6 (xsd:int)

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