About: Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic

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About: Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain. Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain. (en)
Title	Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic (en)
skos:prefLabel	Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic Detection of nongroup A rotaviruses in fecal samples of pigs in the Czech Republic (en)
skos:notation	RIV/00027162:_____/09:#0000488!RIV10-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QF4051), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pšikal, Ivan Smítalová, Radka Rodák, Ladislav Šmíd, Bedřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	309792
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/09:#0000488
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	group B rotavirus; group C rotavirus; nongroup A rotavirus; enteritis; RT-PCR; semi-nested PCR (en)
http://linked.open.../riv/klicoveSlovo	enteritis group B rotavirus group C rotavirus nongroup A rotavirus semi-nested PCR RT-PCR
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[B2D6836349E2]
http://linked.open...i/riv/nazevZdroje	Veterinární medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of biotechnological procedures in the diagnosis and immunoprophylaxis of viral gastroenteritides of swine
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Pšikal, Ivan Rodák, Ladislav Smítalová, Radka Šmíd, Bedřich
http://linked.open...ain/vavai/riv/wos	000264217000002
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0375-8427
number of pages	7 (xsd:int)

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