About: Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP     Goto   Sponge   NotDistinct   Permalink

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Description
  • The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect one MAA source.
  • The detection of IS901 restriction fragment length polymorphism (RFLP) types of Mycobacterium avium subsp. avium (MAA) isolates from different domestic animals between 1998 and 2004 was the aim of this study. Detected RFLP types were compared with previously described RFLP types (the database of the OIE Reference Laboratory for Avian Tuberculosis, Brno, Czech Republic). A total of 62 isolates (56 from pigs, 5 from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using PvuII and PstI endonucleases. Seven PvuII and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Two new PvuII and eight new PstI RFLP types were described. Identical RFLP types were found among isolates originating from individual farms. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in poultry isolates. Detection of identical RFLP types on different farms may reflect one MAA source. (en)
Title
  • Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
  • Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP (en)
skos:prefLabel
  • Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
  • Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP (en)
skos:notation
  • RIV/00027162:_____/09:#0000483!RIV10-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 316221
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/09:#0000483
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • genotyping; mycobacteriosis; avian tuberculosis; molecular epidemiology; zoonosis; database for IS901 RFLP types (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [85FCDDECA69A]
http://linked.open...i/riv/nazevZdroje
  • Veterinární medicína
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 54
http://linked.open...iv/tvurceVysledku
  • Pavlík, Ivo
  • Morávková, Monika
  • Krt, B.
  • Ocepek, M.
  • Pate, M.
http://linked.open...ain/vavai/riv/wos
  • 000268784300002
http://linked.open...n/vavai/riv/zamer
issn
  • 0375-8427
number of pages
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