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Description
| - Two enzyme-linked immunosorbent assays (ELISAs) were tested for their suitability for detecting sulfonamides in wastewater from various stages in wastewater treatment plants (WWTPs), the river into which the wastewater is discharged, and two swine-rearing facilities. Samples from various stages of wastewater purifications gave 0.6-3.1 micro g/L by SMX-ELISA, whereas river samples were 10-fold lower, ranging from below detection to 0.09 micro g/L. The ELISA results in the WWTPs were confirmed by LC-MS/MS, as sulfonamide multiresidue confirmatory analysis identified sulfamethaxazol, sulfapyridine, and sulfasalazine to be present in the wastewater. Sulfamethazine presence at one swine-rearing facility was also confirmed by LC-MS/MS, demonstrating the usefulness of the ELISA technique as a rapid and high-throughput screening method.
- Two enzyme-linked immunosorbent assays (ELISAs) were tested for their suitability for detecting sulfonamides in wastewater from various stages in wastewater treatment plants (WWTPs), the river into which the wastewater is discharged, and two swine-rearing facilities. Samples from various stages of wastewater purifications gave 0.6-3.1 micro g/L by SMX-ELISA, whereas river samples were 10-fold lower, ranging from below detection to 0.09 micro g/L. The ELISA results in the WWTPs were confirmed by LC-MS/MS, as sulfonamide multiresidue confirmatory analysis identified sulfamethaxazol, sulfapyridine, and sulfasalazine to be present in the wastewater. Sulfamethazine presence at one swine-rearing facility was also confirmed by LC-MS/MS, demonstrating the usefulness of the ELISA technique as a rapid and high-throughput screening method. (en)
- Dvě ELISA (enzyme-linked immunosorbent assays) metody byly testovány z hlediska jejich vhodnosti k detekování sulfonamidů v odpadní vodě z různých stadií čištění v čistírnách vody, v řekách do nichž jsou odpadní vody vypouštěny a také ve dvou prostředích vepřínů (swine-rearing facilities) Vzorky z různých stadií čištění odpadní vody vykázaly koncentrace 0.6-3.1 mikro g/L, zatímco říční vzorky byly přibližně 10 krát nižší a to v rozsazích pod 0.6-3.1 mikro g/L. Výsledky ELISA vzorků z čističek vody byly konfirmovány metodou LC-MS/MS, přičemž multiresiduální konfirmační analýza identifikovala ve vzorcích vody sulfamethaxazol, sulfapyridin a sulfasalazin. ELISA byla také konfirmována metodou LC-MS/MS na přítomnost sulfamethazinu v prasečí kejdě. Výsledky demonstrují ELISA techniky jako rychlé a užitečnou vysoko-kapacitní screeningové metody. (cs)
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Title
| - ELISA for sulfonamides and its application for screening in water contamination
- ELISA pro sulfonamidy a její aplikace na screening kontaminace vod (cs)
- ELISA for sulfonamides and its application for screening in water contamination (en)
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skos:prefLabel
| - ELISA for sulfonamides and its application for screening in water contamination
- ELISA pro sulfonamidy a její aplikace na screening kontaminace vod (cs)
- ELISA for sulfonamides and its application for screening in water contamination (en)
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skos:notation
| - RIV/00027162:_____/08:#0000445!RIV09-MZE-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000445
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Analysis; ELISA; LC-MS/MS; residue; sulfonamides (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Agricultural and Food Chemistry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Fránek, Milan
- Rubio, F.
- Shappell, N.
- Shelver, W.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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