About: ELISA for semicarbazide and its application for screening in food contamination

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The development of a direct competitive enzyme-linked immunosorbent assay (ELISA) method based on the derivatization of semicarbazide (SEM) with o-nitrobenzaldehyde and on the immunochemical detection of the derivatited analyte is described. The small aliphatic molecule SEM was coupled to 3-carboxybenzaldehyde to produce carboxyphenyl-SEM (CPSEM), a larger hapten derivative for the generation of specific antibodies. Five rabbits produced antibodies against SEM exhibiting a 50% binding inhibition level (IC 50 value) of 0.06 to 2.28 ug /L in assay buffer. The superior indirect assay based on the antibody MVK39 showed a high dynamic range providing a linear readout in the range of 0.01 to 0.2 ug /L. The optimised ELISA based on MVK31 (IgG) was evaluated using solvent extracted SEM-spiked porcine and baby food puree samples. The development of a direct competitive enzyme-linked immunosorbent assay (ELISA) method based on the derivatization of semicarbazide (SEM) with o-nitrobenzaldehyde and on the immunochemical detection of the derivatited analyte is described. The small aliphatic molecule SEM was coupled to 3-carboxybenzaldehyde to produce carboxyphenyl-SEM (CPSEM), a larger hapten derivative for the generation of specific antibodies. Five rabbits produced antibodies against SEM exhibiting a 50% binding inhibition level (IC 50 value) of 0.06 to 2.28 ug /L in assay buffer. The superior indirect assay based on the antibody MVK39 showed a high dynamic range providing a linear readout in the range of 0.01 to 0.2 ug /L. The optimised ELISA based on MVK31 (IgG) was evaluated using solvent extracted SEM-spiked porcine and baby food puree samples. (en) Práce popisuje vývoj přímé kompetititvní ELISA (enzyme-linked immunosorbent assay) metody založené na derivatizaci semicarbazidu (SEM) with o-nitrobenzaldehyde a imunonochemické detekci tohoto derivatizovaného analytu. Malá alifatická molekula SEM byla kopulována s 3-karboxybenzaldehydem za tvorby karboxyphenyl-SEM (CPSEM), který je větším haptenovým derivatem pro produkci protilátek. Pět králíků produkovalo protilátky proti SEM vykázujíce 50% vazebnou inhibici v pufru na úrovni hodnot IC 50 od 0.06 do 2.28 ug / L. Nejlepší ELISA výsledky (v nepřímém formátu) byla dosažena s protilátkou MVK39, která ukázala vysoký lineární character kalibrace v rozsahu koncentrace 0.01 to 0.2 ug / L. Optimalizovaná ELISA pracující s MVK31 (IgG) protilátkou byla evaluována s použitím fortikovaných extraktů vzorků z hovězího masa a dětské výživy. (cs)
Title	ELISA for semicarbazide and its application for screening in food contamination ELISA pro semicarbazid a její aplikace na screening kontaminace potravin (cs) ELISA for semicarbazide and its application for screening in food contamination (en)
skos:prefLabel	ELISA for semicarbazide and its application for screening in food contamination ELISA pro semicarbazid a její aplikace na screening kontaminace potravin (cs) ELISA for semicarbazide and its application for screening in food contamination (en)
skos:notation	RIV/00027162:_____/08:#0000442!RIV09-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1B53020), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Diblíková, Iva Vass, Maria Černoch, Ivo Fránek, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	365998
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/08:#0000442
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nitrofurans; Hapten design; Semicarbazide detection; ELISA; Antibody specificity; Assay evaluation; porcine samples (en)
http://linked.open.../riv/klicoveSlovo	ELISA Antibody specificity Assay evaluation Hapten design Nitrofurans Semicarbazide detection porcine samples
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[11943CE22F40]
http://linked.open...i/riv/nazevZdroje	Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of antibodies and screening tests for the determination of residues of nitrofuran antibiotics in foods
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	608
http://linked.open...iv/tvurceVysledku	Diblíková, Iva Fránek, Milan Černoch, Ivo Vass, Maria
http://linked.open...ain/vavai/riv/wos	000253088800009
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0003-2670
number of pages	9 (xsd:int)

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