| Attributes | Values |
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| rdf:type
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| Description
| - Robertsonská translokace rob(1;29), spojená s redukcí fertility je široce rozšířená u různých plemen skotu. Laserovou mikrodisekcí, DOP-PCR, klonováním a sekvenováním byla získána vysoce citlivá sonda specifická pro tuto translokaci, která je využitelná pro detekci rob(1;29) v buňkách skotu včetně spermií. S touto sondou a kontrolní sondou pro chromosom 6 bylo analyzováno 1000 spermií od každého z 5 heterozygotních býků - nositelů rob(1;29). Signál rob(1;29)-specifické sondy byl detekován v 48.8%, 50.9%, 50.1%, 51.8%, a 54.8% spermií. Vzorky semene 5 kontrolních býků vykazovaly pouze signál sondy pro chromosom 6. V 1000 spermiích chimerického býka s karyotypem 59,XX,rob(1;29) (57.5%)/ 60,XY (42.5%) v periferních lymfocytech nebyl pozorován signál rob(1;29)-specifické sondy, což demonstruje, že samičí buňky neprocházejí procesem spermatogeneze. (cs)
- The Robertsonian translocation rob(1;29), connected with reduced fertility, is widespread in different cattle breeds. After laser microdissection, DOP-PCR, cloning and sequencing, a highly sensitive translocation-specific DNA probe, suitable for detection of rob(1;29) in cattle cells, including spermatozoa was designed. One thousand sperm from each of five heterozygous translocation carrier bulls were analyzed using this probe and a control probe for chromosome 6. Signals of the translocation-specific probe were detected in 48.8%, 50.9%, 50.1%, 51.8%, and 54.8% of spermatozoa, respectively. Semen samples from five control bulls showed only signals of the control probe for chromosome 6. No sperm head with signal of the translocation-specific probe was observed among 1000 spermatozoa from a chimeric (XX/XY) bull, showing 57.5% of 59,XX,rob(1;29) and 42.5% of 60,XY cells in cultured peripheral lymphocytes, demonstrating that female cells do not pass through the process of spermatogenesis.
- The Robertsonian translocation rob(1;29), connected with reduced fertility, is widespread in different cattle breeds. After laser microdissection, DOP-PCR, cloning and sequencing, a highly sensitive translocation-specific DNA probe, suitable for detection of rob(1;29) in cattle cells, including spermatozoa was designed. One thousand sperm from each of five heterozygous translocation carrier bulls were analyzed using this probe and a control probe for chromosome 6. Signals of the translocation-specific probe were detected in 48.8%, 50.9%, 50.1%, 51.8%, and 54.8% of spermatozoa, respectively. Semen samples from five control bulls showed only signals of the control probe for chromosome 6. No sperm head with signal of the translocation-specific probe was observed among 1000 spermatozoa from a chimeric (XX/XY) bull, showing 57.5% of 59,XX,rob(1;29) and 42.5% of 60,XY cells in cultured peripheral lymphocytes, demonstrating that female cells do not pass through the process of spermatogenesis. (en)
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| Title
| - Detection of translocation rob(1;29) in bull sperm using a specific DNA probe
- Detekce translokace rob(1;29) ve spermiích býků s využitím specifické DNA sondy (cs)
- Detection of translocation rob(1;29) in bull sperm using a specific DNA probe (en)
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| skos:prefLabel
| - Detection of translocation rob(1;29) in bull sperm using a specific DNA probe
- Detekce translokace rob(1;29) ve spermiích býků s využitím specifické DNA sondy (cs)
- Detection of translocation rob(1;29) in bull sperm using a specific DNA probe (en)
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| skos:notation
| - RIV/00027162:_____/08:#0000433!RIV09-MZE-00027162
|
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000433
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - cattle; sperm; translocation carrier; chimera (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - CH - Švýcarská konfederace
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Cytogenetic and genome research
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kubíčková, Svatava
- Rubeš, Jiří
- Černohorská, Halina
- Vozdová, Miluše
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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