| Attributes | Values |
|---|
| rdf:type
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| Description
| - Pstruh duhový byl imunizován DNA vektory kódujícími G a N protein VHSV, aby bylo zjištěno, který z nich indukuje buňkami zprostředkovanou cytotoxicitu (CMC) proti viru virové hemoragické septikémie (VHSV). G protein byl učinnější než N protein. Leukocyty periferní krve (PBL) izolované ze pstruha imunizovaného proti G proteinu zabíjely infikované RTG-2 buňky i xenogenní EPC buňky, což naznačuje účast cytotoxických T lymfocytů i NK buněk. Oproti tomu, PBL ze ptruha imunizovaného proti N proteinu zabíjely pouze infikované RTG-2 buňky, což naznačuje, že tento protein vyvolává pouze CTL odpověď. Toto je první zpráva o cytotoxické imunitní odpovědi u ryb po DNA vakcinaci. (cs)
- To identify viral proteins that induce cell-mediated cytotoxicity (CMC) against viral hemorrhagic septicemia virus (VHSV)-infected cells, rainbow trout were immunized with DNA vectors encoding the glycoprotein G or the nucleocapsid protein N of VHSV. The G protein was a more potent trigger of cytotoxic cells than the N protein. Peripheral blood leukocytes (PBL) isolated from trout immunized against the G protein killed both VHSVinfected MHC class I matched (RTG-2) and VHSV-infected xenogeneic (EPC) target cells, suggesting the involvement of both cytotoxic T lymphocytes (CTL) and NK cells, respectively. In contrast, PBL from trout that were immunized against the N protein only killed VHSV-infected RTG-2 cells, indicating that this protein only elicits a CTL response. This is the first report on cytotoxic immune responses after DNA vaccination in fish.
- To identify viral proteins that induce cell-mediated cytotoxicity (CMC) against viral hemorrhagic septicemia virus (VHSV)-infected cells, rainbow trout were immunized with DNA vectors encoding the glycoprotein G or the nucleocapsid protein N of VHSV. The G protein was a more potent trigger of cytotoxic cells than the N protein. Peripheral blood leukocytes (PBL) isolated from trout immunized against the G protein killed both VHSVinfected MHC class I matched (RTG-2) and VHSV-infected xenogeneic (EPC) target cells, suggesting the involvement of both cytotoxic T lymphocytes (CTL) and NK cells, respectively. In contrast, PBL from trout that were immunized against the N protein only killed VHSV-infected RTG-2 cells, indicating that this protein only elicits a CTL response. This is the first report on cytotoxic immune responses after DNA vaccination in fish. (en)
|
| Title
| - Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus
- Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus (en)
- Buňkami zprostředkovaná imunitní odpověď u pstruha duhového po DNA imunizaci proti viru virové hemoragické septikémie (cs)
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| skos:prefLabel
| - Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus
- Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus (en)
- Buňkami zprostředkovaná imunitní odpověď u pstruha duhového po DNA imunizaci proti viru virové hemoragické septikémie (cs)
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| skos:notation
| - RIV/00027162:_____/08:#0000430!RIV09-MZE-00027162
|
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000430
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Rainbow trout; VHSV; DNA immunization; cell-mediated cytotoxicity; homing (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Developmental and Comparative Immunology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Fischer, U.
- Veselý, Tomáš
- Köllner, B.
- Bergmann, S.
- Dalmo, R.
- Einer-Jensen, K.
- Kock, H.
- Lorenzen, N.
- Ototake, M.
- Schuetze, H.
- Utke, K.
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| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
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