About: On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination

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About: On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cílem studie bylo vyvinout duplexní real time qPCR systémy s interní amplifikační kontrolou specifické pro detekci Mycobacterium avium subsp. paratuberculosis (MAP). Jako detekční cíle byly zvoleny pro kvalitativní analýzu multikopiový IS900 element a pro kvantifikační analýzu jednokopiový F57 element. Citlivost vyvinutých PCR systému a účinnost izolace byly stanoveny pomocí umělé kontaminace nepasterovaného mléka definovanou bakteriální suspenzí a zároveň pomocí plazmidových konstruktů. Vyvinuté systémy byly použity na analýzu. (cs) We developed two duplex real time qPCR systems specific for Mycobacterium avium subsp. paratuberculosis (MAP) detection. These real time qPCR assays amplify the multicopy element IS900 for qualitative analysis and the single copy element F57 for quantitative analysis. Both assays incorporate an internal amplification control. The sensitivity of the developed assays and isolation efficiency were demonstrated through the analysis of raw milk samples artificially contaminated with MAP cells and with plasmids containing cloned fragments of the targets. The developed assays for milk analysis were applied to samples from one farm with two faecal shedding cows. Three hundred and forty five individual milk samples were tested by real time qPCR assays and by cultivation. Hundred and eleven (32.5%) individual milk samples were positive by the real time qPCR, no milk sample was culture positive. The spread of MAP in individual, tank and bulk tank milk samples was also monitored. We developed two duplex real time qPCR systems specific for Mycobacterium avium subsp. paratuberculosis (MAP) detection. These real time qPCR assays amplify the multicopy element IS900 for qualitative analysis and the single copy element F57 for quantitative analysis. Both assays incorporate an internal amplification control. The sensitivity of the developed assays and isolation efficiency were demonstrated through the analysis of raw milk samples artificially contaminated with MAP cells and with plasmids containing cloned fragments of the targets. The developed assays for milk analysis were applied to samples from one farm with two faecal shedding cows. Three hundred and forty five individual milk samples were tested by real time qPCR assays and by cultivation. Hundred and eleven (32.5%) individual milk samples were positive by the real time qPCR, no milk sample was culture positive. The spread of MAP in individual, tank and bulk tank milk samples was also monitored. (en)
Title	On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination Detekce Mycobacterium avium subsp. paratuberculosis v nepasterovaném mléce pomocí IS900 and F57 kompetitivní real time kvantitativní PCR a kultivace (cs) On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination (en)
skos:prefLabel	On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination Detekce Mycobacterium avium subsp. paratuberculosis v nepasterovaném mléce pomocí IS900 and F57 kompetitivní real time kvantitativní PCR a kultivace (cs) On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination (en)
skos:notation	RIV/00027162:_____/08:#0000429!RIV09-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH81065), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Slaná, Iva Králík, Petr Králová, Alena Pavlík, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	384848
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/08:#0000429
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Milk products; Zoonoses; Farmed animals; PCR (en)
http://linked.open.../riv/klicoveSlovo	PCR Farmed animals Milk products Zoonoses
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4C79417C2657]
http://linked.open...i/riv/nazevZdroje	International Journal of Food Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Suppression of paratuberculosis in the CZ: introduction of new methods for the causative agent detection, monitoring of its survival and spread in studs, its distribution in the environment and examination of food and feed contamination.
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	128
http://linked.open...iv/tvurceVysledku	Králová, Alena Králík, Petr Pavlík, Ivo Slaná, Iva
http://linked.open...ain/vavai/riv/wos	000261668100010
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0168-1605
number of pages	8 (xsd:int)

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