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| Description
| - Cílem této práce bylo stanovit, zda mastné kyseliny s krátkým (SCFA) a středně dlouhým řetězcem (MCFA) mohou ovlivnit expresi virulence u Salmonella Typhimurium, snížit kolonizaci střevního traktu prasat salmonelami a jejich vylučování trusem. Proto byly nejprve určeny hodnoty MIC pro 4 SCFA (k. mravenčí, octová, propionová a máselná) a 2 MCFA (%22caproic a caprylic acid%22). Exprese genu hilA a invaze do prasečích tkáňových kultur byla výrazně nižší pokud byly baktérie kultivovány v LB půdě obsahující sub-MIC koncentrace k. propionové a máselné (obě při 10mM koncentraci), caproic nebo caprylic acid (obě při 2mM koncentraci). Pokud se tyto kyseliny podávaly infikovaným prasatům v kapalné formě, pak neměly žádný vliv na kolonizaci střevního traktu prasat a vylučování trusem. Pokud však byly podávány v enkapsulované formě jako aditivum do krmiva, takovýto přípravek vedl u infikovaných prasat ke snížení ke snížení vylučování salmonel trusem. (cs)
- The aim of the present study was to determine whether short- (SCFA) and medium-chain fatty acids (MCFA) are able to alter virulence gene expression and to decrease Salmonella Typhimurium colonization and shedding in pigs. Minimal inhibitory concentrations (MIC) of 4 SCFA (formic acid, acetic acid, propionic acid, butyric acid) and 2 MCFA (caproic and caprylic acid) were determined using 54 porcine Salmonella Typhimurium field strains. Expression of hilA and invasion in porcine intestinal epithelial cells was significantly lower when bacteria were grown in LB-broth containing sub-MIC concentrations of butyrate or propionate (10mM) and caproic or caprylic acid (2mM). When given as feed supplement to pigs experimentally infected with Salmonella Typhimurium, coated butyric acid was shown to be effective in decreasing the levels of faecal shedding and intestinal colonization. Uncoated fatty acids, however, did not influence fecal shedding, intestinal or tonsillar colonization in pigs.
- The aim of the present study was to determine whether short- (SCFA) and medium-chain fatty acids (MCFA) are able to alter virulence gene expression and to decrease Salmonella Typhimurium colonization and shedding in pigs. Minimal inhibitory concentrations (MIC) of 4 SCFA (formic acid, acetic acid, propionic acid, butyric acid) and 2 MCFA (caproic and caprylic acid) were determined using 54 porcine Salmonella Typhimurium field strains. Expression of hilA and invasion in porcine intestinal epithelial cells was significantly lower when bacteria were grown in LB-broth containing sub-MIC concentrations of butyrate or propionate (10mM) and caproic or caprylic acid (2mM). When given as feed supplement to pigs experimentally infected with Salmonella Typhimurium, coated butyric acid was shown to be effective in decreasing the levels of faecal shedding and intestinal colonization. Uncoated fatty acids, however, did not influence fecal shedding, intestinal or tonsillar colonization in pigs. (en)
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| Title
| - Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs
- Mastné kyseliny ovlivňují virulenci Salmonella Typhimurium a snižují její schopnost kolonizovat střevní trakt prasat (cs)
- Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs (en)
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| skos:prefLabel
| - Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs
- Mastné kyseliny ovlivňují virulenci Salmonella Typhimurium a snižují její schopnost kolonizovat střevní trakt prasat (cs)
- Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs (en)
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| skos:notation
| - RIV/00027162:_____/08:#0000416!RIV09-MZE-00027162
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000416
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Salmonella Typhimurium; pig; colonization; SCFA; MCFA (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Rychlík, Ivan
- Volf, Jiří
- Ducatelle, R.
- Haesebrouck, F.
- Pasmans, F.
- Boyen, F.
- Dewulf, J.
- Van Immerseel, F.
- Vanparys, A.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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