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| rdf:type
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| Description
| - Předmětem studie bylo charakterizovat vliv estrálního cyklu na zisk, morfologii a meiotickou kompetenci prasečích oocytů. Cyklující prasničky byly použity jako donorky oocytů. Donorky byly poraženy v časném, středním a pozdním stadiu luteální nebo folikulární fáze potom, co jejich estrální cyklus byl synchronizován. Oocyty byly izolovány ze středně velkých nebo malých folokulů, kultivovány ve zracím médiu po dobu 47 hodin, fixovány a barveny. Zisk, morfologie a meiotická kompetence se lišily u oocytů získaných ze středně velkých a malých folikulů nezávisle na fázi cyklu. Výsledky experimentů potvrdily, že populace oocytů se mění pokud se týká kvantity, morfologické kvality a meiotické kompetence v závislosti na stádiu folikulárního vývoje. Zdá se, že pozdní luteální a časná folikulární fáze jsou efektivnější pro získávání oocytů, protože více morfologicky normálních oocytů s vyšší meiotickou kompetencí bylo získáno ve srovnání s ostatními stadii estrálního cyklu. (cs)
- The objective of the study was to characterize the effect of the estrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. For the experiments, cycling gilts were used as oocyte donors. They were slaughtered at the early, middle and late stages of luteal or follicular phase after their estrous cycle was synchronized. Oocytes were isolated from medium or small follicles, matured for 44 hours, fixed and stained. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competence regardless of the phase. It can be concluded that a porcine oocyte population changes in terms of quantity, morphological quality and meiotic competence according to the stage of follicular development. The late luteal and early follicular phases appeared to be most productive for oocyte recovery, because more morphologically normal oocytes with greater meiotic competence was collected, as compared with the other stages of the estrous cycle.
- The objective of the study was to characterize the effect of the estrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. For the experiments, cycling gilts were used as oocyte donors. They were slaughtered at the early, middle and late stages of luteal or follicular phase after their estrous cycle was synchronized. Oocytes were isolated from medium or small follicles, matured for 44 hours, fixed and stained. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competence regardless of the phase. It can be concluded that a porcine oocyte population changes in terms of quantity, morphological quality and meiotic competence according to the stage of follicular development. The late luteal and early follicular phases appeared to be most productive for oocyte recovery, because more morphologically normal oocytes with greater meiotic competence was collected, as compared with the other stages of the estrous cycle. (en)
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| Title
| - Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro
- Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro (en)
- Fáze říjového cyklu ovlivňuje morfologii a zrání prasečích oocytů in vitro (cs)
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| skos:prefLabel
| - Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro
- Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro (en)
- Fáze říjového cyklu ovlivňuje morfologii a zrání prasečích oocytů in vitro (cs)
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| skos:notation
| - RIV/00027162:_____/08:#0000361!RIV09-MZE-00027162
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000361
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Gilts; follicular development; oocytes; morphology; meiotic competence (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hulínská, Pavlína
- Machatková, Marie
- Hanzalová, Kateřina
- Horáková, Jindra
- Rečková, Zuzana
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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