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| rdf:type
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| Description
| - Efficiency of in vitro embryo production is highly variable amongst individual sires in cattle.To eliminate that this variability is not due to sperm chromatin damage caused by separation or capacitation,chromatin integrity was evaluated.Sperm before and after separation,after 3-h and 6-h capacitation or incubation were evaluated by SCSA.Based on changes in the nonDFI-sperm proportion,sires were categorized as DNA-unstable, DNA-stable and DNA-most stable bulls.In DNAus and DNAs bulls,separation produced the increase of the mean nonDFI-sperm proportion,as compared with value before separation.Capacitation produced the decrease in the mean nonDFI-sperm proportion only in DNAus bulls.In DNAms bulls,neither separation nor capacitation had any effect on the mean nonDFI-sperm proportion. It can be concluded that,although separation and capacitation may produce some changes in sperm chromatin integrity,these are not associated with different in vitro fertility of the bulls involved.
- Efficiency of in vitro embryo production is highly variable amongst individual sires in cattle.To eliminate that this variability is not due to sperm chromatin damage caused by separation or capacitation,chromatin integrity was evaluated.Sperm before and after separation,after 3-h and 6-h capacitation or incubation were evaluated by SCSA.Based on changes in the nonDFI-sperm proportion,sires were categorized as DNA-unstable, DNA-stable and DNA-most stable bulls.In DNAus and DNAs bulls,separation produced the increase of the mean nonDFI-sperm proportion,as compared with value before separation.Capacitation produced the decrease in the mean nonDFI-sperm proportion only in DNAus bulls.In DNAms bulls,neither separation nor capacitation had any effect on the mean nonDFI-sperm proportion. It can be concluded that,although separation and capacitation may produce some changes in sperm chromatin integrity,these are not associated with different in vitro fertility of the bulls involved. (en)
- Cílem práce bylo hodnotit integritu chromatinu motilních spermií individuálních býků a charakterizovat stav chromatinu během kapacitace stimulované heparinem. Vzorky spermií byly odebrány před separací po separaci, po 3 a 6h.inkubaci a kapacitaci a vyšetřeny metodou SCSA Podle reakce spermií na separaci a kapacitaci byly býci rozděleni do 3 skupin: DNA nestabilní, stabilní a vysoce stabilní býky. Separace zvýšila podíl nonDFI-spermií u DNAus a DNAs býků. U DNAus býků se podíl nonDFI-spermií během kapacitace snižoval a zjištěné rozdíly byly statisticky průkazné. Separace neovlivnila podíl nonDFI-sperm u DNAms-býků a tento podíl se nezměnil i po celou dobu kapacitace spermií. Lze konstatovat, že separace spermií pozitivně ovlivnila podíl spermií s neporušenou strukturou chromatinu. Reakce spermií na heparin byla specifická pro individuální býky a nedošlo při ní k výraznější degradaci chromatinu. Nebyl prokázán vztah mezi integritou chromatinu a plodností býků in vitro. (cs)
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| Title
| - Evaluation of chromatin integrity of motile bovine spermatozoa capacitated in vitro
- Charakteristika integrity chromatinu v populaci motilních spermií býků kapacitovaných in vitro (cs)
- Evaluation of chromatin integrity of motile bovine spermatozoa capacitated in vitro (en)
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| skos:prefLabel
| - Evaluation of chromatin integrity of motile bovine spermatozoa capacitated in vitro
- Charakteristika integrity chromatinu v populaci motilních spermií býků kapacitovaných in vitro (cs)
- Evaluation of chromatin integrity of motile bovine spermatozoa capacitated in vitro (en)
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| skos:notation
| - RIV/00027162:_____/08:#0000342!RIV09-MZE-00027162
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1B44018), Z(MZE0002716201)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000342
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Bulls; Spermatozoa; Chromatin integrity; In vitro capacitation (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Rybář, Roman
- Hulínská, Pavlína
- Machatková, Marie
- Horáková, Jindra
- Rečková, Zuzana
- Máchal, L.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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