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rdf:type
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Description
| - Bylo zjištěno, že oocyty získané od prepubertálních prasniček mají sníženou vývojovou kompetenci a vyšší frekvenci aneuploidních oocytů oproti dospělým prasnicím. (cs)
- Oocytes derived from prepubertal gilts show reduced developmental competence when compared to oocytes collected from adult sows. Therefore, the aim of the study was to investigate whether gilts (4-5 months old) and adult sows (average age 3.5 years) of the same breed differ with regard to the rate of chromosomally unbalanced oocytes after IVM. COCs derived from individual pairs of slaughterhouse ovaries, were matured in vitro and analyzed cytogenetically by conventional staining (Giemsa) and FISH methods. Altogether, 72 females (31 sows, 41 gilts) and 430 secondary oocytes (194 and 236 oocytes of sows and gilts, respectively) were investigated. Cytogenetic analysis revealed diploid (Giemsa, FISH) and aneuploid (FISH) spreads. The incidence of diploid oocytes was similar for sows (26.0%) and gilts (24.5%) whereas the rate of aneuploid oocytes (nullisomic/disomic) was 8-times higher in gilts (10.8%) than in sows (1.3%). Diploid and aneuploid oocytes were observed in 64% of investigated females.
- Oocytes derived from prepubertal gilts show reduced developmental competence when compared to oocytes collected from adult sows. Therefore, the aim of the study was to investigate whether gilts (4-5 months old) and adult sows (average age 3.5 years) of the same breed differ with regard to the rate of chromosomally unbalanced oocytes after IVM. COCs derived from individual pairs of slaughterhouse ovaries, were matured in vitro and analyzed cytogenetically by conventional staining (Giemsa) and FISH methods. Altogether, 72 females (31 sows, 41 gilts) and 430 secondary oocytes (194 and 236 oocytes of sows and gilts, respectively) were investigated. Cytogenetic analysis revealed diploid (Giemsa, FISH) and aneuploid (FISH) spreads. The incidence of diploid oocytes was similar for sows (26.0%) and gilts (24.5%) whereas the rate of aneuploid oocytes (nullisomic/disomic) was 8-times higher in gilts (10.8%) than in sows (1.3%). Diploid and aneuploid oocytes were observed in 64% of investigated females. (en)
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Title
| - Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly
- Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly (en)
- Prepubertální prasničky a dospělé prasnice produkují stejné množství diploidních oocytů in vitro, ale incidence aneuploidií je vyšší u prasniček (cs)
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skos:prefLabel
| - Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly
- Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly (en)
- Prepubertální prasničky a dospělé prasnice produkují stejné množství diploidních oocytů in vitro, ale incidence aneuploidií je vyšší u prasniček (cs)
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skos:notation
| - RIV/00027162:_____/07:#0000240!RIV08-MZE-00027162
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/07:#0000240
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - chromosomal imbalance; oocyte; IVM; pig; FISH (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Rubeš, Jiří
- Lechniak, D.
- Pers-Kamczyc, E.
- Antosik, P.
- Sosnowski, J.
- Warzych, E.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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